Microbiology: An Introduction
Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Chapter 6, Problem 2CAE

Heat lamps are commonly used to maintain foods at about 50°C for as long as 12 hours in cafeteria serving lines. The following experiment was conducted to determine whether this practice poses a potential health hazard.

Beef cubes were surface-inoculated with 500,000 bacterial cells and incubated at 43–53°C to establish temperature limits for bacterial growth. The following results were obtained from heterotrophic plate counts performed on beef cubes at 6 and 12 hours after inoculation:

Chapter 6, Problem 2CAE, Heat lamps are commonly used to maintain foods at about 50C for as long as 12 hours in cafeteria

Draw the growth curves for each organism. What holding temperature would you recommend? Assuming that cooking kills bacteria in foods, how could these bacteria contaminate the cooked foods? What disease does each organism cause? (Hint: See Chapter 25.)

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A batch of turkey rolls (10 lb—approximately 4.5 Kg—each) were cooked to 165°F internal temperature in bags, opened, sliced, vacuum-packaged, and stored at 40°F. The product was expected to have a refrigerated shelf life of 50 days. However, after 40 days, the packages contained gas and approximately 107 bacterial cells/g of meat. The bacterial species involved in the spoilage was found to be Leuconostoc carnosum, which is killed at 165°F. What could be the sources of the bacterial species in this cooked product?
Two flasks of E. coli are grown in batch culture in the same medium (2% glucose and amino acids; no nitrate) and at the same temperature (378C). Culture #1 is well aerated. Culture #2 is anoxic. After 16 hours the following observations are made: ■ Culture #1 has a high cell density; the cells appear to be in stationary phase, and the glucose level in the medium is reduced to 1.2%. ■ Culture #2 has a low cell density; the cells appear to be in logarithmic phase, although their doubling time is prolonged (over 1 hour). The glucose level is reduced to 0.2%. Why does culture #2 have so little glucose remaining relative to culture #1, even though culture #2 displayed slower growth and has less biomass?
starting with four bacterial cells per milliliter in a rich nutrient medium, with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in 1 ml of this culture after 10 hours?

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Microbiology: An Introduction

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cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY