Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
expand_more
expand_more
format_list_bulleted
Videos
Textbook Question
Chapter 6, Problem 1CAE
Clinical Applications and Evaluation
1. Assume that after washing your hands, you leave ten bacterial cells on a new bar of soap. You then decide to do a plate count of the soap after it was left in the soap dish for 24 hours. You dilute 1 g of the soap 1:106 and plate it on heterotrophic plate count agar. After 24 hours of incubation, there are 168 colonies. How many bacteria were on the soap? How did they get there?
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Worksheet 2. Draw it.
Instruction: A bacterial culture was in log phase. At time x, an antibacterial compound was added
to the culture. Draw the lines indicating addition of a bacterial compound and a bacteriostatic
compound. Explain why the viable count does not immediately drop to zero at x?
Time
Log of number
of cells
Fit to
3. A group of students wants to study the effects of temperature on bacterial growth. To get bacteria, they
leave petri dishes of nutrient agar open on a shelf. They then put the dishes in different places: an
incubator (37 degrees C) a lab room (21 degrees C), a refrigerator (10 degrees C) and a freezer (0 degrees
C). Bacterial growth is measured by estimating the percentage of each dish covered by the bacteria at the
end of a three day growth period.
a) What is the independent variable?
b) What is the dependent variable?
c) List two (or more) ways to improve this experimental design.
have a progressive su
week.
1. Why are basic stains the most commonly used stains in
microbiology?
2. What does the term "aseptic" mean?
3. Why are differential stains used more often than simple
stains?
4. What are the three parts of a stain?
5. Why is it critical to allow cells to air dry before heat
fixation?
6. Give an example of a basic stain and an acidic stain.
7. Give an example of a differential stain.
8. What is a medium?
9. What is the definition of a bacterial colony?
10. Why do we want to get an isolated bacterial colony from a
mixed culture?
Next lab: A continuation of staining techniques.
to
a
Chapter 6 Solutions
Microbiology: An Introduction
Ch. 6 - Prob. 1RCh. 6 - Prob. 2RCh. 6 - Prob. 3RCh. 6 - Seven methods of measuring microbial growth were...Ch. 6 - By deep-freezing, bacteria can be stored without...Ch. 6 - A pastry chef accidentally inoculated a cream pie...Ch. 6 - Nitrogen and phosphorus added to beaches following...Ch. 6 - Differentiate complex and chemically defined...Ch. 6 - DRAW IT Draw the following growth curves for E....Ch. 6 - NAME IT A prokaryotic cell hitched a ride to Earth...
Ch. 6 - Two culture media were inoculated with four...Ch. 6 - Two culture media were inoculated with four...Ch. 6 - Use the following graph to answer questions 3 and...Ch. 6 - Use the following graph to answer questions 3 and...Ch. 6 - Assume you inoculated 100 facultatively anaerobic...Ch. 6 - The term trace elements refers to a. the elements...Ch. 6 - Which one of the following temperatures would most...Ch. 6 - Which of the following is not a characteristic of...Ch. 6 - Which of the following types of media would not be...Ch. 6 - An organism that has peroxidase and superoxide...Ch. 6 - E. coli was incubated with aeration in a nutrient...Ch. 6 - Clostridium and Streptococcus are both...Ch. 6 - Most laboratory media contain a fermentable...Ch. 6 - Flask A contains yeast cells in glucose-minimal...Ch. 6 - Clinical Applications and Evaluation 1. Assume...Ch. 6 - Heat lamps are commonly used to maintain foods at...Ch. 6 - The number of bacteria in saliva samples was...
Additional Science Textbook Solutions
Find more solutions based on key concepts
The correct term for production of offspring. Introduction: Reproduction is an important life process for most ...
Biology Illinois Edition (Glencoe Science)
Some people compare DNA to a blueprint stored in the office of a construction company. Explain how this analogy...
Biology: Concepts and Investigations
The term ‘spore’.
Biology Science Notebook
Why is it necessary to be in a pressurized cabin when flying at 30,000 feet?
Anatomy & Physiology (6th Edition)
Propose a model for the assembly of a flagellum in a typical Gram-positive cell envelope.
Prescott's Microbiology
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Answer TRUE OR FALSE only. 1. All types or kinds of media powder has a standard ratio of 23:1000. Meaning that 1000 ml of water is needed to dissolve 23 grams of powder medium. 2. Simple stains use one dye and cannot differentiate various types of bacteria while differential stains use several dyes in order to differentiate between different kinds of bacteria. 3. A certain student prepared his fixed sample for examination by pouring Nigrosin which is an acidic stain. He then observed that under the microscope the background was stained but the bacterial cells were untouched. This student therefore adapted the acid-fast staining procedure. 4. In doing serial dilutions, the original sample must be shaken at least 25 times to obtain a uniform distribution of organisms. 5. Label all reagents with its name, concentration and date of its preparation except for water.arrow_forward1. TRUE or FALSE. Relative to a 24 hour culture of endospore forming bacteria, a 72 hour culture would contain more endospores.arrow_forward3- Visible Growth in Tubes Growth in Petri Dishes 0 µg/ml 2 µg/ml 0 1 3 4 ÖÖÖÖÖÖÖÖ 2 5 6 7 number 1, and After addition of The figure given above summarizes an experiment carried out to test the efficacy of an antimicrobial substance. In the tube number 0, no antimicrobial substance was added into bacterial culture. 2 µg/ml of antimicrobial substance was added into tube the amount of antimicrobial was doubled for each consecutive tube. antimicrobial into tubes, tubes were incubated for overnight and after incubation tubes were observed for growth. The growth status of bacteria in each tube was shown as (+) and (-). In addition to observation of visible growth, 1 ml of sample from each tube was collected and plated into petri dishes and incubated for additional overnight. Similarly, the growth status in petri dishes was as (+) and (-). According to given information; a. Determine the minimum inhibitory concentration (MIC). b. Determine the minimum bactericidal concentration (MBC). c.…arrow_forward
- Helping tags: Biology, Food Microbiology, Spore formers, sulfide spoilage spores 25 g of sugar sample was obtained from the production line for analysis of raw ingredients. It was diluted to 250 ml and was heated at 100 deg Celsius for 5 minutes. After cooling, 20 ml of the sample solution was divided equally among 6 tubes of sulfite agar. After incubation, the following counts were obtained: 35, 26, 29, 32, 34, and 31. Compute for the number of sulfide spoilage spores and express the count per 50 g of sugar. . . . WILL UPVOTE, just pls help me answer the question and show COMPLETE solution.arrow_forwardSubculture and Colony Morphology Descriptions Following identification of the Gram-negative isolate and Gram-positive isolate, you next subculture each onto fresh nutrient agar plates. Briefly describe the subculture process in three or so sentences and what this allowed you to achieve; follow this with colony morphology descriptions of each. Description: Isolate A – Describe: Colony morphology: Medium & incubation temperature: Isolate B – Describe: Colony morphology: Medium & incubation temperature:arrow_forwardMicrobes in our Lives 1. List several ways in which microbes affect our lives. Naming and Classifying Microorganisms 1. Recognize the system of scientific nomenclature that uses two names: a genus and a specific epithet. 2. Differentiate the major characteristics of each group of microorganisms. 3. List the three domains. A Brief History of Microbiology 1. List at least four beneficial activities of microorganisms. 2. Name two examples of biotechnology that use recombinant DNA technology and two examples that do not. 3. Explain the importance of observations made by Hooke and van Leeuwenhoek. 4. Compare spontaneous generation and biogenesis. 5. Identify the contributions to microbiology made by Needham, Spallanzani, Virchow, and Pasteur. 6. Define bacteriology, mycology, parasitology, immunology, and virology. 7. Explain the importance of microbial genetics and molecular biology.arrow_forward
- TRUE or FALSE only! 1. We need to look at the body tube’s side when focusing to prevent the breakage of the microscope glass slide. 2. In transferring samples of bacteria from one medium to another growth medium a sterilized inoculating loop is used. 3. In drying leaf samples for a thesis experiment, the proper laboratory instrument to be used is the autoclave.arrow_forwardWork N2. Initial isolation of pure culture anaerobic microorganisms from mixture of bacteria soil suspension o blood agar anaerostatearrow_forwardBONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J Karrow_forward
- method used in lab? we will be using the filter disk method. Which is NOT true of the filter disc A zone of inhibition is an area of no growth around the filter disc. You will apply an agent, such as a particular mouthwash, to the filter disc. You should apply the mouthwash to a plate that has been already been swabbed by the bacteria by pipetting one drop of the mouthwash or rinse directly onto the plate. A zone of inhibition indicates that an agent that has been applied to the filter disc either inhibited or killed the organism on the spread plate.arrow_forwardAnglais Question 9. With three examples, explain how co- culture could contribute to the growth of uncultivable bacteria?arrow_forwardTEST III. MATCHING TYPE. Match the microorganisms in column A with their corresponding product in column B, then match your answer in column B to column C.arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Bacterial Endospore Formation -Biology Pundit; Author: Biology Pundit;https://www.youtube.com/watch?v=6_sinRhE8zA;License: Standard YouTube License, CC-BY