Microbiology: An Introduction
Microbiology: An Introduction
11th Edition
ISBN: 9780321733603
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: Benjamin Cummings
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Chapter 6, Problem 1A

E. coli was incubated with aeration in a nutrient medium containing two carbon sources, and the following growth curve was made from this culture.

  1. a. Explain what happened at the time marked x.
  2. b. Which substrate provided “better’ growth conditions for the bacteria? How can you tell?
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What are the defining characteristics of the exponential/log phase of the bacterial growth curve.  Explain the culture conditions that arise that cause the phase to end.   In different culture media, and the exact same starting inoculum, will the end of exponential/log occur at the same time?  Why or why not?  A graph or a drawing of the growth curve would help but is not essential to answer this question.
A microbiology student was given a mixed culture of two different gram positive bacteria species to grow into a culture medium using correct aseptic techniques. After two days,one gram positive bacterial species grew on the media and the growth appeared red on the surface of the medium. Tthe second gram positive bacterial species grew and the growth appeared yellow on the surface of the medium. What is the possible explanation for the differences in the color of the bacterial growth? A. the culture was contaminated   B. the microbiologist put too much inoculum on the culture medium   C. the medium was a selective medium   D. the medium was differential
If the generation time of Escherichia coli is 30 minutes, starting with 4 E. coli cells, how many cells can you obtain after 2 hours of growth? A: cells

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Microbiology: An Introduction

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cell culture and growth media for Microbiology; Author: Scientist Cindy;https://www.youtube.com/watch?v=EjnQ3peWRek;License: Standard YouTube License, CC-BY