1) Enhanced GFP protein has: An excitation peak at 488nm and emits maximally at 507nm. An excitation peak at 488nm and emits maximally at 587nm An excitation peak at 488nm and emits maximally at 567nm. An excitation peak at 458nm and emits maximally at 507nm.   2) A typical sequencing read will yield _____ nucleotides of unambiguous sequence. 1800-2000 3800-4000 2800-3000 800-1000   3) Sequencing primers should be designed to bind where? ~ 25 nucleotides upstream of the beginning region to be sequenced. ~ 25 nucleotides downstream of the beginning region to be sequenced ~ 60 nucleotides upstream of the beginning region to be sequenced. ~ 60 nucleotides downstream of the beginning region to be sequenced

Q: You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa 100 kDa -80 kDa 80 kDa…

A: you have asked multiple question please repost the question and mention the sub parts needed to be…

Q: Give at least two common loading control used in Western Blotting?

A: Western blotting is a laboratory technique that is used to detect a specific protein in a a…

Q: You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa -100 kDa -80 kDa 80…

A: Blotting, as we all know, is a process that uses electrophoresis to separate DNA, RNA, and protein…

Q: This BLAST program performs the greatest number of searches for the same query sequence and database…

A: BLAST is used in bioinformatics an an algorithm which compares the sequences of amino acids and of…

Q: What is the minimum and maximum cDNA input from FFPE samples in library preparation phase to perform…

A: Next genome sequencing is quickly turning into the technique for decision for transcriptional…

Q: Explain the origin of the signal that is used in the nucleic acid sequencing method known as…

A: Ion torrent technology is a modern genome sequencing method that converts chemically encoded…

Q: consider the DNA segment with a sequence: 3'-TACGGTACGGGATTG-5'. if the given DNA sample was…

A: Ion Torrent sequencing is also known by the name of: Ion semiconductor sequencing pH-mediated…

Q: To clone a bacterial lipase gene into the cloning vector pET28a, you know the full sequence of the…

A: For the ease of manipulation, vectors must be relatively small molecules. They must be capable of…

Q: Cloning vector, vị, is 90 kbps (kilobase pairs) long, and contains two cleavage sites recognized by…

A: Restriction enzyme - restriction enzyme, also called restriction endonuclease, is a protein produced…

Q: Using the table below, what volume from the master mix will be dispensed to each sample tube if the…

A: Answer:  As we know, C1V1= C2V2, where C1 and C2 = initial and final concentrations V1 and V2 =…

Q: Consider the DNA segment with a sequence: 3'-TACGGTACGGGATTG-5' If the given DNA sample was…

A: Ion torrent sequencing : The ion torrent sequencing techniques makes use of the principle that when…

Q: You have a very concentrated sample of DNA which you need to dilute for sequencing 1:6000 with a…

A: Dilution is defined as the ratio of volume from stock to the final volume.  Dilution= Stock volume /…

Q: Outline how much of each of these compounds you would weigh out in order to make a 250 mL stock of…

A: TE buffer is used for many process among which Agarose gel electrophoresis is one where it is used.

Q: in your word, identify one difference one similarity between 16 S sequencing and metagenomic shotgun…

A: With the advancements in science, different techniques have emerged to sequence the genomes of…

Q: Although a number of different animals have been successfully cloned, the process of creating cloned…

A: A living organism, which is genetically identical to that of the other organism is known as a Clone.…

Q: Explain why DNA ladders are usually included during gel electrophoresis.

A: BASIC INFORMATION  GEL ELECTROPHORESIS  It is a process in which the DNA fragments are separated…

Q: As seen in the illustration below, you inserted your desired gene into pBR322. You used BamHI to…

A: A cloning vector like the pBR322 plasmid has some important features - an origin of replication,…

Q: How would you simply explain Protein Analysis (Western blotting)?

A: Proteins, also known as polypeptides, are made up of amino acids. They’re large, complex molecules…

Q: CPT code assignment 44970

A: Cpt code is current procedural terminology. These are the numerical codes helps to identify the…

Q: If the DNA duplex for the beta chain of haemoglobin represented by the sequence…

A: Given the template strand in transcription unit is 3'-TACCACGTGGACTGAGGACTCCTCTTCAGA-5' If the…

Q: How much 340bp insert DNA should be added to a ligation in which 50ng of 4.3kb vector will be used?…

A: The question is based upon the concept of molarity (number of moles of a compound per litre of…

Q: Compare sequencing method 1 Illumina and sequencing method PacBio for: a. Read length. What are the…

A: Illumina sequencing methods are also called short read methods as they read shorter sequences in a…

Q: An RFLP marker is located 1 million bp away from a gene of interest. Your goal is to start at this…

A: Molecular mapping is used to find the location of gene on the chromosome and also the distance…

Q: The estimated size of fusion protein of an antibiotic (540bp)  and an anti-inflammatory agent (630…

A: Size of antibiotic is 540bp. It will code for 180 amino acids. This is calculated by 540/3 = 180,…

Q: Sanger sequencing originally used 4 lanes in gels. These lanes represented sequences of different…

A: Sanger sequencing is a method of DNA sequencing based on the selective incorporation of…

Q: At higher amounts of protein, the Bradford assay is not linear. Consider the plot to the right: what…

A: Bradford assay is used to determine the unknown concentration of proteinbin a sample from standard…

Q: Please answer ASAP RNA sequencing (RNA-Seq) of one sample in one run of a massively parallel…

A: Next-generation sequencing (NGS) is a massively parallel sequencing technology that offers…

Q: For the given restriction enzyme: BslI (CCNNNNN^NNGG)  i) Approximately how many fragments would…

A: Restriction endonucleases are enzymes that does cleavage of DNA at specific sites. These specific…

Q: Discuss the main similarities and differences in the nucleic acid sequencing methods known as…

A: DNA sequencing is the process of determining the sequence of nucleic acids, which are the basic…

Q: In reversible terminator sequencing, how would the sequencing process be affected if the…

A: The basic between sanger's sequencing and reversible terminator sequencing is that Sangar's based on…

Q: Starting with an AA, 2n = 14 genome of Triticum urartu (wild einkorn wheat) and a BB, 2n = 14…

A: Cultivated wheats refer mainly to two types: the hexaploid bread wheat (Triticum aestivum)…

Q: Assume 2x108 reads of 75 bps long are obtained from a next-generation sequencing experiment to…

A: Next-generation sequencing relies on the continuous sequencing of the genome parts and later…

Q: In the extraction of DNA from a Banana, alcohol is added to the filtrate after filtration. What will…

A: Extraction of DNA from Banana:  Bananas are excellent source of dna for extraction because cells of…

Q: a student uses a monoclonal antibody to detect a protein using a direct Western-blotting protocol…

A: Secondary antibodies are used to detect a target after a particular primary antibody has been bound…

Q: Where do you expect to see multiple “Ns” within a sequencing read? a)Within the first 25…

A: Nucleotide sequences have their role in every aspect of genetic machinery. This includes…

Q: A 22-kb piece of DNA has the following restriction sites:A batch of this DNA is first fully digested…

A: The restriction enzymes clean the nucleotide fragment at specific sides called the restriction…

Q: In DNA isolation techniques, a washing step is always done prior to the final resuspension. What is…

A: DNA isolation: The process of extraction of DNA may be broken down into five primary phases that…

Q: Sanger sequencing originally used 4 lanes in gels. These lanes represented sequences of different…

A: This method makes use of the mechanism of DNA synthesis by DNA polymerase  DNA polymerase requires…

Q: The best molecular technique to quantify the gene transcripts is (write in fu!!).

A: The Central Dogma concept states that "DNA makes RNA and RNA makes protein". This concept is…

Q: a) What are vectors? Describe extensively the roles vectors play in genetic engineering? Write short…

A: Note: Solving answer only for part A as per the guidelines, repost remaining part, please. The…

Q: To achieve a 10x depth coverage for a genome of length 10 Mbp, how many reads of 100 bp are…

A: A Coverage is multiplier based on  total size of the genome. Formula =  Coverage = (read length) x…

Q: A. RNA seq data is genrated by illumina sequencing. But before this step, what molecule is isolated…

A: Sequencing is the method of determining the sequence of a biomolecule. RNA is a type of nucleic acid…

Q: What size bands would you expect to see on the Southern blots using probe 1 and probe 2 for an…

A: Introduction The term Sickle cell disease defines the condition where due to a mutation, the blood…

Q: In the human genome for the beta chain of haemoglobin (the oxygen-carrying protein in the red blood…

A: Need to find the complementary strand for the given strand.

Q: Match each type of variant with the best technique that could be used to detect the variant. (Hint:…

A:

Q: You inserted your gene of interest in pBR322 as illustrated below. You spliced the vector and insert…

A: Screening The procedure to identify and select the individual of interest.