Sanger sequencing originally used 4 lanes in gels. These lanes represented sequences of different lengths obtained by adding: One of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP) to the reaction vials; one for each lane All of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP), together with all of the 4 deoxynucleotides (DATP, dGTP, DCTP and dTTP), to all of the reaction vials One of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP) to the reaction vials; one for each lane, together with all of the 4 deoxynucleotides (DATP, DGTP, DCTP and dTTP) in each vial All of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP) to the reaction vials; together with one of the 4 deoxynucleotides (DATP, DGTP, dCTP and dTTP), one for each lane, in each vial.
Sanger sequencing originally used 4 lanes in gels. These lanes represented sequences of different lengths obtained by adding: One of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP) to the reaction vials; one for each lane All of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP), together with all of the 4 deoxynucleotides (DATP, dGTP, DCTP and dTTP), to all of the reaction vials One of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP) to the reaction vials; one for each lane, together with all of the 4 deoxynucleotides (DATP, DGTP, DCTP and dTTP) in each vial All of the 4 dideoxynucleotides (ddATP; ddGTP; ddCTP; ddTTP) to the reaction vials; together with one of the 4 deoxynucleotides (DATP, DGTP, dCTP and dTTP), one for each lane, in each vial.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Sanger sequencing originally used 4 lanes in gels. These lanes represented sequences of different lengths obtained by adding:
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