You inserted your gene of interest in pBR322 as illustrated below. You spliced the vector and insert using BamHI. After ligation and transformation, describe your screening procedures. Scal 3844 Pvul 3733 Pstl 3607 Vspl 3537 Eco311 3433 Eam11051 3361 Cail 2884. rep (pMB1) bla (Ap) 3293 Bsul5l 23 EcoRI, Xapl 4359, Hindi 29 Eco321 185 Aatil 4284, Sspl 4168 3133 Afill, Pscl 2473 2519 Lgul 2350/ 4153 pBR322 4361 bp Ndel 2295/ Bst11071 2244/ 2106 1915 Bspol, Nhel 229 rop Pull 2064 Pfol 2117 Esp31 2122 Ppu211 2225 Psyl 2217 BamHI 375 Tstl 389 SgrAl 409 tet (Tc) 1276 Pael 562 Xagl 622 Sall 651 Boxl 712 Eco521 939 Bsp681 972 Bvel 1063 Mva12691 1353 Eco1301 1309 Eco881 1425 Misl 1444 Bpu101 1580 Bsgl 1650 Kpn21 1664 BseJl 1668

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