The process which occurs at a temperature range of 92-95 °C in a PCR reaction. Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

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Answer 1

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Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

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In a typical PCR reaction what is happening in stages occurring at temperature ranges (a) 92–95°C, (b) 45–65°C, and (c) 65–75°C.

The final amount of each primer required in a PCR reaction is 25 picomol. If the total volume of the PCR reaction is equal to 100 µl and the stock concentration of each primer is equal to 0.0025 mM. Calculate the volume of stock primer that needs to be added in order to ensure a primer amount of 25 picomol.

Your amplicon from PCR was subjected to AGE for analysis. You are shown the image of the gel loaded with the following lanes: (A) negative control, (B) size ladder (1, 2, 3, 4, 6, and 10 kb), (C) gDNA extract, (D) PCR amplicon. However, due to mishaps while loading the gel with the samples, you are not sure which lane is which. You are shown a diagram of the obtained gel below. a. Label each lane of the gel. Write only the corresponding letters in the wells above. b. Above each band in the size ladder, write its size (in kb). c. Approximate the size (in kb) of the polystyrenase gene. Write your answer above the band corresponding to the gene. Bonus: If you wish to identify the bacterial species in this scenario, what gene is most commonly and routinely sequenced? Answer: __________________________________

Answer 2

Question

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

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Answer 3

Question

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

Expert Solution & Answer

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Answer 4

Question

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

Expert Solution & Answer

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Answer 5

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

Answer 6

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Answer 7

Chapter 20, Problem 19PDQ

(a)

Summary Introduction

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Answer 8

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

Answer 9

(b)

Summary Introduction

To determine: The process which occurs at a temperature range of 45-65 °C in a PCR reaction.

Introduction: The PCR has two limitations; to synthesize primers; some information about the nucleotide sequence of target DNA must be known. The other is minor contamination of the sample can cause problems.

Answer 10

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

Answer 11

(c)

Summary Introduction

To determine: The process which occurs at a temperature range of 65-75 °C in PCR reaction.

Introduction: The PCR has many advantages such as it is an important tool for detecting bacteria and viruses. The technique is advantageous for studying the samples from single cells, fossils, and the crime scene.

Answer 12

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Answer 13

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Answer 14

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Answer 15

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The process which occurs at a temperature range of 92-95 °C in a PCR reaction. Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Solution Summary: The author explains the polymerase chain reaction, which produces multiple copies of a specific DNA segment.

Videos

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Want to see the full answer?

Chapter 20 Solutions

The process which occurs at a temperature range of 92-95 °C in a PCR reaction. Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Solution Summary: The author explains the polymerase chain reaction, which produces multiple copies of a specific DNA segment.

Videos

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction. Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.

Want to see the full answer?

Chapter 20 Solutions

To determine: The process which occurs at a temperature range of 92-95 °C in a PCR reaction.

Introduction: The polymerase chain reaction is a method used to produce multiple copies of a specific DNA segment. Thousands to millions copies of a specific DNA fragment are produced by using PCR.