Chemistry In Context
Chemistry In Context
9th Edition
ISBN: 9781259638145
Author: Fahlman, Bradley D., Purvis-roberts, Kathleen, Kirk, John S., Bentley, Anne K., Daubenmire, Patrick L., ELLIS, Jamie P., Mury, Michael T., American Chemical Society
Publisher: Mcgraw-hill Education,
Question
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Chapter 14, Problem 25Q

(a)

Interpretation Introduction

Interpretation:

Structure of Luminol and the various functional groups present in the chemical structure has to be given.

Concept introduction:

Functional group: They are certain substitutes in the organic molecules which determine the characteristic reactions taking place in it.

Different types of functional groups are present and it includes alkane, alcohol, aldehyde, amine, ether, carboxylic acid etc.

Primary and secondary amine:

Primary amine refers to the functional group that contains one nitrogen atom bonded to two hydrogen atoms and one carbon containing group and the secondary amine contains nitrogen bonded to one hydrogen atom and two carbon containing groups.

Carbonyl group: The functional group that contains carbon atom which is doubly bonded with the oxygen atom.

(b)

Interpretation Introduction

Interpretation:

Component of blood that catalyzes the fluorescence of Luminol has to be given.

Concept introduction:

Luminol:

  • It is a solid which is soluble in polar organic solvents.
  • It is important in forensic studies since it can be used to determine trace amounts of blood at crime scenes.
  • Luminol on reaction with iron in the blood produces a blue glow.

(c)

Interpretation Introduction

Interpretation:

The purpose of the oxidizing agent in the Luminol solution has to be given.

Concept introduction:

Luminol:

  • It is a solid which is soluble in polar organic solvents.
  • It is important in forensic studies since it can be used to determine trace amounts of blood at crime scenes.
  • Luminol on reaction with iron in the blood produces a blue glow.

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True or false? A. Phosphorescence occurs at higher energy then fluorescence. B. Fluorescence occurs at longer wavelengths than phosphorescence. C. Fluorescence occurs at shorter wavenumbers than phosphorescence.
A protein’s concentration can be determined spectrophotometrically by mixing it with an indicator called a Bradford reagent and measuring the amount of light absorbed by the protein. Use the plot below to determine the mass of protein (in micrograms, μμg ) in 100.0 mL of the solution if its absorbance is 0.398. A.  52 B.  3.1 C.  2.4 D.  0.52 E.  0.031
3. In a fluorescence spectrum, what quantities are plotted on the x- and y-axes? In a calibration curve for a fluorescence experiment, what quantities are plotted on the x- and y-axes? 4. Explain the difference between an excitation spectrum and an emission spectrum. Why does the excitation spectrum occur at lower wavelengths than the emission spectrum? 5. What is the main difference in the spectra resulting from atomic emission and molecular emission? 6. Describe how you would use serial dilution to prepare 100 mL of a 0.50 ppm QS solution from a 10 ppm stock QS solution.
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