Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 13, Problem 6TY
Summary Introduction
Introduction:
The genetic material is all the living organism is the DNA (deoxyribonucleic acid). The DNA sequences are unique to the organisms. The changes in the DNA sequence of an organism is referred to as mutation. The mutagen are the substances capable of causing mutations in the cells.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy. Explain what they are used for. Also mention the actual biological function of the respective enzymes.
1) RNaseH
A graduate student who has not taken BIOL 519 thinks they have discovered a new oncogene that could cause breast cancer. The student transfects cells with this oncogene and finds that the cells proliferate
uncontrollably. The student next designs a PCR assay that will detect the presence of the oncogene in cancerous breast tissue. The student sets up the assay and analyzes the presence of the oncogene in
normal and tumor issue samples (See data below). The student is disappointed to see that the potential oncogene is present at similar levels in both the normal and tumor tissue. Therefore, the student concludes
that this gene is not an oncogene. Do you agree with this conclusion? Justify your answer (5-6 senetences max).
Oncogene?
GADPH
Normal
Tumor 1
Tumor 2
Tumor 3
A graduate student who has not taken BIOL 519 thinks they have discovered a new oncogene that could cause breast cancer. The student transfects cells with this oncogene and finds that the cells proliferate uncontrollably. The student next designs a PCR assay that will detect the presence of the oncogene in cancerous breast tissue. The student sets up the assay and analyzes the presence of the oncogene in normal and tumor issue samples (See data below). The student is disappointed to see that the potential oncogene is present at similar levels in both the normal and tumor tissue. Therefore, the student concludes that this gene is not an oncogene. Do you agree with this conclusion? Justify your answer (5-6 senetences max).
Chapter 13 Solutions
Principles of Biology
Ch. 13.1 - Prob. 1CCCh. 13.1 - Prob. 2CCCh. 13.1 - Prob. 1TYKCh. 13.1 - Prob. 2TYKCh. 13.2 - Prob. 1CCCh. 13.2 - Prob. 2CCCh. 13.2 - Prob. 1TYKCh. 13.2 - Prob. 2TYKCh. 13.3 - Prob. 1CCCh. 13.3 - Prob. 1TYK
Ch. 13.4 - Prob. 1CCCh. 13.4 - Prob. 1BCCh. 13.4 - Prob. 2CCCh. 13.4 - Prob. 3CCCh. 13.4 - Prob. 4CCCh. 13.4 - Prob. 1TYKCh. 13.4 - Prob. 2TYKCh. 13 - A mutation removes a single base pair within the...Ch. 13 - Prob. 2TYCh. 13 - Prob. 3TYCh. 13 - Prob. 4TYCh. 13 - Prob. 5TYCh. 13 - Prob. 6TYCh. 13 - Prob. 7TYCh. 13 - Prob. 8TYCh. 13 - Prob. 9TYCh. 13 - Prob. 10TYCh. 13 - Prob. 1CCQCh. 13 - Prob. 2CCQCh. 13 - Prob. 3CCQCh. 13 - Prob. 1CBQCh. 13 - Prob. 2CBQ
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.arrow_forwardBoth cloning and PCR can be used for making copies of DNA. What is the advantage or limitation of one over the other?arrow_forwardThe enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy. Explain what they are used for. Also mention the actual biological function of the respective enzymes. T7 RNA polymerase reverse transcriptase RNaseHarrow_forward
- Consider the following results from the Ames test: Test plate - 20 colonies Does the test plate contain a chemical that is a mutagen? O Not enough information is present to answer the question. Yes O Noarrow_forwardThe following statements are true about common gene cloning procedures except: -DNA plasmids can help move around genes into other cells - Restriction enzymes are very important for cutting up and linking chunks of DNA -We make it so that genes from plants or animals are expressed in bacteria so the products can be harvested - DNA plasmids are chunks of chromosomal DNA used for cloningarrow_forwardFill in the following with the correct words about cloning (the first blank will be the first word and the second blank will be the second word. There are two types of cloning, the first is when scientists change the cellular function this is called cloning and can help with creating insulin and vaccines to name a few. The other type is when DNA is extracted from a somatic cell and is used to replace DNA in an egg, this is called cloning. O gene, organism O organism, gene O specific, general O micro, macroarrow_forward
- The idea behind PCR-based diagnostics is that a very small number of microbial genomes in a patient sample can be multiplied by PCR and more easily detected by the clinical team managing the patient’s care. Also, genetic-based diagnostics are very useful for viral infections because we don’t have biochemical tests, etc. to distinguish one virus from another (remember, viruses are metabolically inactive). However, a lot of work goes into the development of these tests. For instance, PCR requires primers that are complementary to the viral genome that is being copied. If primers are complementary to the target genome, what must scientists know to design primers that bind to the viral genome to be copied? (I mean this to be a general question; don’t look up the details of designing primers)arrow_forwardWith the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining different stages of the cloning process that involves the following:a. Isolation of target DNA fragments (often referred to as inserts)b. Ligation of inserts into the plasmid, creating recombinant molecules c. Transformation of recombinant plasmids into bacteria or other suitable host for propagationd. Screening/selection of hosts containing the intended recombinant plasmid. For this stage(d), discuss the importance of a second marker that can be used for screening of genomic DNA for colonies containing the pka-1 under the principle of insertional inactivation. This should be properly explained using all the attributes of the plasmid described above.arrow_forwardFill the Table with mutagenic agents and provide their type (physical, chemical, biological) and their classification based on their effect (teratogenic, carcinogenic, clastogenic, or non-specific), together with their modes of action. Mutagen Type of Mutagen Classification based on effect Action UV – radiation X-Ray radiation Virus Heat 5-bromouracil Ethidium bromide 2-aminopurine Acridine orange Proflavine Cobalt Nickel Methylhydrazine Temozolomide Ethyl ethane sulfatearrow_forward
- An insertion of two nucleotides into a gene results in a frameshift mutation. This statement is: True Falsearrow_forwardDescribe how P1vir transduction can be used to introduce a gene mutation into E. coli. Use your own diagrams to aid your answer. Remember to refer to the figure in the text. You should describe / show both the molecular biology and the transduction procedure. Tip: BioRender is excellent to create figures.arrow_forwardWhich of the following most accurately describes the process of DNA cloning? set of laboratory procedures that consist of cutting a segment of DNA with restriction enzymes set of laboratory procedures that uses living cells to mass-produce specific DNA fragments set of laboratory procedures by which a DNA fragment is transferred from a living organism to a SNP chip the manipulation of DNA fragments in a laboratory using modern techniques of molecular biology set of laboratory procedures that consist of isolating of a DNA fragment from a living organism and inserting it into a plasmidarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage LearningBiology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage Learning
Human Heredity: Principles and Issues (MindTap Co...
Biology
ISBN:9781305251052
Author:Michael Cummings
Publisher:Cengage Learning
Biology Today and Tomorrow without Physiology (Mi...
Biology
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cengage Learning
Molecular Techniques: Basic Concepts; Author: Dr. A's Clinical Lab Videos;https://www.youtube.com/watch?v=7HFHZy8h6z0;License: Standard Youtube License