Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN: 9781305251052
Author: Michael Cummings
Publisher: Cengage Learning
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Textbook Question
Chapter 13, Problem 10QP
Cloning Genes Is a Multistep Process
Which enzyme is responsible for covalently linking DNA strands together?
- a. DNA polymerase
- b. DNA ligase
- c. EcoRl
- d. restriction enzymes
- e. RNA polymerase
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Chapter 13 Solutions
Human Heredity: Principles and Issues (MindTap Course List)
Ch. 13.5 - Do you think the way this issue was handled should...Ch. 13.5 - Prob. 2EGCh. 13.7 - If you were offered the chance to have the genome...Ch. 13.7 - Prob. 2EGCh. 13 - Improving the nutritional value of food has long...Ch. 13 - Improving the nutritional value of food has long...Ch. 13 - Prob. 3CSCh. 13 - What Are Clones? Cloning is a general term used...Ch. 13 - Prob. 2QPCh. 13 - Prob. 3QP
Ch. 13 - Prob. 4QPCh. 13 - Prob. 5QPCh. 13 - Prob. 6QPCh. 13 - Cloning Genes Is a Multistep Process The following...Ch. 13 - Prob. 8QPCh. 13 - Prob. 9QPCh. 13 - Cloning Genes Is a Multistep Process Which enzyme...Ch. 13 - Cloning Genes Is a Multistep Process In cloning...Ch. 13 - Prob. 12QPCh. 13 - Prob. 13QPCh. 13 - Prob. 14QPCh. 13 - Prob. 15QPCh. 13 - Cloned Libraries You are running a PCR to generate...Ch. 13 - Prob. 17QPCh. 13 - Prob. 18QPCh. 13 - Prob. 19QPCh. 13 - Analyzing Cloned Sequences A base change (A to T)...Ch. 13 - Prob. 21QPCh. 13 - Analyzing Cloned Sequences What kind of...Ch. 13 - Prob. 23QP
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- What carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reactionarrow_forwardWhich DNA polymerase in prokaryotes is involved in the replacement of primers with deoxynucleotides? a.DNA-Pol I b.DNA-Pol II c.DNA-Pol Ill d.DNA-Pol IVarrow_forwardWhich type of cells were used to extract the DNA that was sequenced? a. red blood cells c. white blood cells b. intestinal epithelium d. cheek swab What type of mutation caused Nicholas’s disease? a. frameshift c. nonsense b. missense d. insertionarrow_forward
- What is the purpose of the low temperature step in the PCR reaction? a. To allow DNA polymerase to synthesize new DNA in the 3' to 5' direction b. To permanently deactivate DNA polymerase c. To allow primers to anneal to DNA templates d. To allow DNA polymerase to synthesize new DNA in the 5' to 3' directionarrow_forwardPCR is a technique used to synthesize DNA fragments. Select all the reagents needed for PCR to occur. A. DNA template B. Thermo stable DNA polymerase C. Two primers D. Type I endonucleasesarrow_forwardWhich of the following is not a part of the Sanger method tosequence DNA?a. dideoxynucleotides b. DNA polymerase c. electrophoresis d. reverse transcriptasearrow_forward
- A primer is laid down complementary to the DNA sequence TAGCAATCGCA to prime synthesis of a daughter strand. What will the sequence of the primer be? A. ATCGTTAGCGT B. ATGGTTAGGGT C. AUCGUUAGCGU D. AUCGTUAGCGTarrow_forwardPart A: During cloning, the DNA is cut with a restriction enzyme giving it what? A. more introns B. sticky ends C. a polyA tail D. a binding site for a transcription factor Part B: If the scientist wanted to have a large number of copies of the gene for use in further study, which technique would the scientist use? A. PCR B. epigenetics C. hybridization D. gel electrophoresis Part C: If this gene were found to be expressed at different levels in different cells of the same organism, what would be responsible? A. transcription factor B. rRNA C. ribosome D. RNA polymerase Part D: If a disease were identified as being caused by defects in the cytochrome c gene, then the copy isolated could be used for what? A. PCR B. gene therapy C. stem cell work D. DNA fingerprintingarrow_forwardWhat is the correct sequence action of proteins during the process of DNA replication? A. DNA polymerase, helicase, primase, ligase, SSB B. helicase, SSB, primase, ligase, DNA polymerase C. ligase, helicase, SSB, primase, DNA polymerase D. helicase, SSB, primase, DNA polymerase, ligasearrow_forward
- What will most likely be the effect of the change in the DNA molecule? * A. the change will cause a harmful mutation B. the DNA molecule will be unable to replicate C. the organism will be reproduce D. the DNA molecules will code for a different protein Original strand: T A C G T A T G A A G C, Mutant strand: T A G C T A T G A A G C. what type of mutation? * A. Substitution B. Deletion C. Insertion D. Nonsense Which mechanism contributes to accuracy during DNA Replication? * A. The mismatch repair system recognizes an incorrect base-pair and corrects the mistake in the non-methylated strand B. Using primers increase accuracy because the first nucleotides is a new nucleic acid chain are more like to be correct. C. all DNA polymerase have a 5'-3' exonuclease activity which can remove…arrow_forwardChoose the combination of answers that most accurately completes the statement.The function of ligase is to a. rejoin segments of DNA c. synthesize cDNA b. make longitudinal cuts in DNA d. break down ligamentsarrow_forwardWhat is DNA polymerase? a.An enzyme that carries out DNA replication b.Short, single strand of DNA that base-pairs with a specific DNA sequence c.An enzyme that corrects mutations that arise during the replication of DNA d.An enzyme that seals any gaps that remain between bases of replicating strands of DNAarrow_forward
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