Write a note on different culturing, metabolic and physiological techniques used in the Microbiology Lab for culturing as well as identification of microorganisms.
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A: Given: When culturing microorganisms, you must ensure you work to prevent unwanted contamination.…
Q: uestions What is the purpose of the soft agar? 1
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Q: Describe how to make bacterial culture in different media. Explain different cultural techniques…
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Q: Enumerate and describe briefly the components of a culture medium and indicate their uses
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Q: Oven sterilization of aqueous culture medium. Effect: Rationale:
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Q: Draw two tubes showing how you have inoculated microorganisms in urease and in nutrient agar
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Q: Turbidometry is a method used for counting microbes?
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Q: Complete the table to contrast EMB Agar and MSA.
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Q: Should you sample an area for microbial growth before or after sanitization? Eplain why.
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Q: Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions…
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Write a note on different culturing,
Step by step
Solved in 7 steps with 3 images
- Describe how you would execute this first stage from the point you are handed the nutrient broth tube containing the mixed culture, through to the appearance of two colony types on a nutrient agar plate. Assume you have all the necessary equipment and materials at your disposal. Be concise, but thorough; limit yourself to a short paragraph (1/4-1/2 page at most) – include the method and techniques; what you expect to see, and how you would avoid contamination during the process.Provide a brief description and state the function of the following tools and equipment used in the microbiology laboratory. Incubator Hot air oven Centrifuge Spectrophotometer Magnetic stirrer Orbital shakerProvide a brief description and state the function of the following tools and equipment used in the microbiology laboratory. o Commmon glassware ( test tubes, culture tubes and screw capped tubes ) o Petri dish o Pipette o Glass spreader o Inoculation needle o Inoculation/transfer loop o Bunsen burner o Pressure cooker o Incubator o Hot air oven o Centrifuge o Spectrophotometer o Magnetic stirrer o Orbital shaker o Hot plate o Water bath o Carbon dioxide cylinder o Single pan balance with weights o Chemical balance o Ph meter o Colony counter o Electrophoretic apparatus o autoclave
- In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with moldDescribe the step by step method of preparing culture media in microbiology.Why is it important to become familiar with the microbiology laboratory's apparatus and equipment?
- List the two main types of culture media and how each differs and the types of microbes which grow in each type.Describe how to make bacterial culture in different media. Explain different cultural techniques with an example. LONG DETAILED ANSWER NEEDED. I WILL RATE.Briefly discuss the following topic and include appropriate examples where necessary. Physiological characteristics employed in microbial classification.
- Identify one hazard and one associated risk present in an actual microbiology laboratory.Describe the purpose of identifying unknown bacterial culture in medical field. Name the step by step experiments/procedures that microbiologists follow in order to identify microorganisms.Assuming you are working in a microbiology laboratory and a client sent you a sample for the following analyses: Identification of all microorganisms in the soil sample Enumeration of culturable bacteria in the sample Enumeration of both culturable and non-culturable bacteria in the sample For a to c, explain the following: Method of analyses to be used Step by step report on how the analyses will be conducted