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Use of streak plate technique to isolate and purify psychrotrophic spoilage-causing bacteria
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- Why are the streak plates incubated at 7°C when selecting for psychrotrophic bacteria?selective agar media for gram-positive bacteria?In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?
- Which culturing method provided the clearest results, the semisolid medium tube or the soft agar plate? Explain.Explain why the media where the Staphylococcus aureus is plated has turned yellow but the media where Staphyloccocus epidermidis plated is still pink? The media used would be the Mannitol Salt Agar Medium.Write a note on different culturing, metabolic and physiological techniques used in the Microbiology Lab for culturing as well as identification of microorganisms.