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- Tell me three things about how cultures are handled in a clinical laboratory.Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:5) Aseptic technique refers to A) the microbial inoculum placed into a test tube or onto a Petri plate. B) a series of practices to avoid contamination. C) the autoclave and other sterilizing procedures. D) cleanliness in the laboratory.
- Preservatives are added to the following pharmaceutical products EXCEPT Select one: Ophthalmic solutions Injections in eye tissues Non-sterile Ointments Multi-dose sterile productsWhy are specimens for bilirubin testing protected from light? Discuss handling procedures for bilirubin samples.In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropper
- List five (10) sites on a patient from which potential pathogens could be isolated and prepared for staining and microscopic identification.Salmonella-Shigella (SS) agar plate results (kitchen sink drain and kitchen dish cloth) Using the following information about SS agar and the image below, describe and identify the growth on the SS agar plate in the table on page 6. SS agar plate - results of kitchen sink drain and kitchen dish cloth (plate below) Salmonella-Shigella (SS) agar: Salmonella-Shigella agar (SS agar) is a selective and differential medium utilized for the selection and characterization pathogenic enteric bacteria. SS agar contains lactose, bile salts, ferric citrate, and neutral red. Kitchen The bile salts select for Gram-negative bacteria, while inhibiting Gram-positive bacteria. sink drain The other components differentiate among Gram-negative bacteria. Coliform bacteria such as E. coli will ferment the lactose in the media, resulting in bacterial growth with a pink color. (They do not produce any hydrogen sulfide.) Members of the genus Salmonella will not ferment lactose, but do product hydrogen sulfide…These are examples of the 5 rules of asepsis, except: 1. Any item used for patient care or that is visibly soiled must be considered "dirty" and "contaminated" 2. If the item has been cleaned with a detergent disinfectant, it is both clean and decontaminated 3. Sterile items are devices processed by steam, dry heat, ethylene oxide or other sterilization method to kill most organisms 4. Dirty, clean and sterile items must be separated at all times
- Item 1. Describe the BEST method of sterilization or treatment to reduce pathogens to safe level for each of these 5 items. 2. Explain why this is the best method and why it is BETTER than other possible methods. (Specific treatments were mentioned in lecture for each item.) Plastic milk containers 1. Preserving low-acid vegetables to kill Clostridium botulinum endospores. 2. Liquids contaminated with prions (list BOTH methods of sterilization discussed in lecture) 3. Skin 4. Media with heat sensitive nutrients 5.Solid and liquid media are commonly prepared based on manufacturer’s instructions. If no instruction is provided on how the media should be sterilized, what would be the best option? METHOD:RATIONALE:What will pose an ethical issue in these procedures are performed - sterilization?