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procedure/s in performing aseptic transfer of bacterial cultures in (include illustration)
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- procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar plate to agar slant.procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (2) agar slant culture to agar slantIn performing the Kirby-Bauer procedure in a clinical laboratory setting, why must the agar be a certain depth? Why must the absorbance of the inoculum be standardized?
- In aseptic technique, what will happen if the inoculated solid plated media is not inverted prior to incubation?during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.a. Explain whether or not any of the methods in fi gure 2.9 could beused to determine the total number of cells present in a patient’s specimen.b. After performing the streak plate method on a bacterial specimen, theculture was incubated for 48 hours at 37°C. Upon viewing the plate, therewas heavy growth (with no isolated colonies) in the fi rst quadrant, but nogrowth was apparent in the remaining quadrants. Please discuss errors in the procedure that could have produced this result.
- Blood agar is often used to observe changes in the appear-ance of the agar around the colonies growing on this medium.This medium could then be called:(a) Selective(b) Designated(c) Differential(d) Defined(e) ExactWhy it is possible/ applicable to use recrystallization and vacuum filtration with Luminol? Cite some reasons.(Explain in at least 1 paragraph)The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate 1 10 Plate 2 10 Plate 3 10 dilution dilution dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 10 dilution -6 *Too many to count Number of colony forming units (CFU) TMTC* TMTC* 840 28 19 1
- The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution Plate 3 107 dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 382 83 10 2 0What is the physical appearance (solid, liquid, semi-solid) of following media: agar plateYou are tasked to observe the motility of an UNKNOWN Bacteria isolated from a mixed culture, which technique/s is BEST to perform? How shall the procedure be carried out? Explain.