Q: Explain the reasons you would use a negative stain?
A: In a negative stain the slide is actually stained. This is because the negatively charged stain…
Q: What are the sources of error in performing Gram staining?
A: Bacteria can be divided into two categories namely gram positive(with thin layer of peptidoglycan)…
Q: You receive a bacterial culture in the lab and your job is to figure out information which will be…
A: Best option to stain a bacterial culture is Gram Stain Method. It is used to determine gram status…
Q: Crystal violet is an example of what type of stain?
A: A stain is used to clearly view a specimen under the microscope. For staining, a smear of the…
Q: Why must heat or a surface-active agent be used with application of the primary stain during…
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin
A: The living cells can be seen under a microscope. The cells is not visible properly as it is…
Q: What is gram staining and its purpose? Why is it only applicable in bacteria?
A: Introduction: Differential staining methods like Gram staining, acid-fast staining, endospore…
Q: Why are basic dyes more effective for bacterial staining than acidic dyes?
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: Why is the Gram stain considered a differential stain?
A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…
Q: What is the difference between simple stain and complex stain? Describe the color of an S. aureus…
A: Most types of cells don't have a normal pigment and are in this way challenging to see under the…
Q: Which step is the most crucial or most likely to cause poor results in the Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: In four steps, describe the simple staining procedure.
A: A stain is used to clearly view a specimen under the microscope. In simple staining, bacteria are…
Q: What is a differential stain? why is it used?
A: Differential stain is a type of stain use to identify organisms.
Q: Give the principle of acid fast staining and gram staining.
A: Microorganisms are extremely small in size and hence cannot be visualized directly. Specialized…
Q: What is the difference between simple stains anddifferential stains?
A: Simple stains : It generally make all of the organisms in a sample appear to be the same color even…
Q: Why is it sometimes difficult to attain both good resolution and good contrast at the same time for…
A: Magnification and resolution are important because the magnification in larges the image and the…
Q: What are the principle and basic concepts of NEGATIVE staining?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: What are the most commonly used methods in staining? why?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a tiny scale.…
Q: How are negative stains prepared?Name two applications for which this type of stain especially…
A: Negative staining is defined as a method of demonstrating the type of small objects (like bacteria)…
Q: Describe how simple staining and fluorescence staining are similar and how they are different. What…
A: Microscopy is a technical field for the use of microscopes to visualize objects and locations of…
Q: What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method?
A: Kinyoun staining is a technique used to stain bacteria. Specifically, acid-fast species of the…
Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used
A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…
Q: What step normally associated with staining bacterial cells is omitted when the dimensions of cells…
A: Step 1 Gram staining is a technique of staining that is used to differentiate and categorize…
Q: What is an example of a negative stain?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: Briefly discuss the difference between simple and differential staining? Which is better suited for…
A: Staining is a laboratory technique in which various coloured dyes are used to highlight the specimen…
Q: What's the theory and conclusion of a acid fast stain?
A: It was Ziehl who originally devised differential staining procedures, that were later refined by…
Q: Which type of stain would be the best choice for negative staining of most bacterial cells: a basic…
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: Give three situations where the negative staining procedure would be used.
A: Negative staining is a specimen preparation technique that requires an acidic dye such as India Ink…
Q: Why are chemical stains required for visualizing cells and tissues with the basic light microscope?
A: Cell staining is a technique that is used to better visualize cells and cell components under a…
Q: How does viability staining differ from stains like DAPI?
A: Staining is the technique in biology, which increases the visibility at the microscopic level. Dyes…
Q: Differential stains function to
A: The widely used staining method in the microbiology is the differential staining that are gram…
Q: About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this…
A: Gram staining technique is a differential test based on the composition of bacteria cell wall. It…
Q: What is the difference between Giemsa and Wright stain?
A: Staining is a technique used to enhance contrast in samples, generally at the microscopic level.…
Q: Which step in the Gram stain procedure is most prone to error? If done incorrectly, how might that…
A: The differential stain is used to distinguish between different structures and cellular components.…
Q: What major advantage does phase-contrast microscopy haveover staining?
A: Phase-contrast microscopy is an optical microscopy technique used in cells. When the light passes…
Q: What is a crystal violet stain and its purpose?
A: Crystal violet is that the name given to the cluster of similar organic compounds that are used as…
Q: Why do we need to stain microorganisms?
A: Microbiology is the study of microbes. Microorganisms include bacteria, fungus, archaea, and…
Q: When performing a negative stain, how does it work and why does it only stain the background?
A: * Negative stain is practice of applying heavy metal salt stain to sample for observation hence…
Q: Draw what you would expect to see for acid fast stain and endospore stain procedure if you did not…
A: only acid fast appears red/pink. Only endospore appears green. Vegetative cell become colourless.
Q: bacteria require staining before microscopic examination?
A: Bacteria are single-celled organisms with a small size. Bacteria can be found practically everywhere…
Q: In staining, why is there a need for contrasting stains?
A: Answer
Q: k there are 2 staining reagents applied in differential s
A: Differential Staining- It is a staining process which uses more than one staining reagent. it is…
Q: do we need to stain microorganisms?
A: The study of microorganisms is termed microbiology. Microorganisms is microorganism, fungi, archaea…
Q: In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the…
A:
Q: What type of chromophore is associated with a negative stain?
A: Bacteria are unicellular prokaryotic organisms that maintain a definite shape since they possess the…
Q: How can the thickness of the smear affect the staining procedure?
A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…
Q: Name two fixatives used in staining?
A: Histology is the study of ultrastructure of tissues using thin sectioning techniques like microtomy,…
Is it possible to perform double or triple staining using fluorescent dyes? Explain.
Step by step
Solved in 2 steps
- What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?Describe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple stain, and differential stain. please provide minimum of 400 words
- Why is it necessary to use contrasting stains while staining?Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?a: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.
- Which step is the most crucial or most likely to cause poor results in the Gram stain?During staining, the first step is to dewax the slide. What is the purpose of the step? What is the effect of insufficient dewaxing?What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain, what is the mordant?