Draw what you would expect to see for acid fast stain and endospore stain procedure if you did not stain with counterstain. Color pencils need to be used.
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Draw what you would expect to see for acid fast stain and endospore stain procedure if you did not stain with counterstain. Color pencils need to be used.
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- Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methodsIs acid fast staining differential stain , if yes, what are the 2 types of stain usedWhat is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide? Paragraph BIU
- During staining, the first step is to dewax the slide. What is the purpose of the step? What is the effect of insufficient dewaxing?Which of the following are true about a differential stain. There may be more than one answer! No chemicals are needed. More than one stain is used The acid fast stain is an example The Gram stain is an example.Complete for Additional Differential Staining B: Negative staining ~ thin smear C: Endospore Stain
- The first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stainThe acid-fast stain is used to stain _____. The decolorizer used to destain the cells is ______. Positive cells that retain the primary stain are ______, while negative cells are counterstained with _____ and will have a ______ color. *Please use answers from the options provided in the image below.Choose the 4 most correct steps from the list below for a simple positive stain and organize them into the most correct order. Briefly explain the purpose of each step you chose. • Heat fix • Dip slide in acid alcohol • Wash slide with ethanol • Apply Congo Red to the slide • Apply Crystal Violet to the slide • Make a smear • Wash slide with water
- Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain affect the staining of bacteria? Can dyes other than methylene blue be used for direct staining? Briefly explain.Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct) a acid alcohol b crystal violet c carbolfuschin d malachite green e safranin f iodine g alcohol h methylene blue