Q: What kind of stain would a pathologist use to visualise a chrondrosarcoma under the microscope? why…
A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Biological…
Q: What is the usual stain used for staining a sputum smear?
A: Essentially, sputum comprises of a combination of saliva and mucus. additionally named as phlegm, It…
Q: is it essential that the primary stain and counterstain be of contrasting colors
A: Gram staining is one of the crucial type of differential straining .It divides bacteria into two…
Q: Define fluorescent staining.
A: The advent of microscopy started with the development of optical microscopy. Then the level of…
Q: Why is heat necessary in spore staining?
A: BASIC INFORMATION SPORULATION It is a process during which spores are formed. We can find it in…
Q: in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?
A: The Ziehl-Neelson (ZN) stain is the most commonly utilized of the different types of AF staining…
Q: What are Romanowsky stains? Name four of these.
A: Staining is a technique used to enhance contrast in samples , generally at the microscopic level.…
Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?
A: bacteria like Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…
Q: What are the principle and basic concepts of DIFFERENTIAL staining?
A: The simple dye is used in the technique of simple staining to highlight only the specific structures…
Q: Why is there a need to use a staining agent in observing the specimen?
A: Staining agent: The things that owes-out stains were recognized as staining agent,…
Q: Is the flagellar stain a Positive or Negative Simple Stain protocol?
A: Staining is a technique that is used to enhance contrast in samples, generally at the microscopic…
Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…
A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…
Q: Differentiate between negative and positive staining, givingexamples.
A: These are the staining methods that are used to see bacteria under a microscope.
Q: Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methods
A: Ziehl neelsen staining principle The presence of mycolic acids in the cell walls of acid-fast…
Q: Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can…
A: According to the guidelines we have to answer first 2-3 question rest you can ask separately thank…
Q: What is the difference between simple stains anddifferential stains?
A: Simple stains : It generally make all of the organisms in a sample appear to be the same color even…
Q: What is the significance of heat fixing the smear?
A: Gram staining is a common laboratory technique in microbiology that is used to differentiate…
Q: Application of differential staining technique.
A: Differential staining is a staining technique that employs the application of several different…
Q: What would happen if you had skipped the heat fixing step and continued with the staining procedure?
A: There are various staining methods are used for staining the bacteria, namely simple staining,…
Q: If you conducted an acid-fast stain on a sputum sample from a person with tuberculosis, what would…
A: The acid-fast staining technique is a differential staining technique. The bacteria which get…
Q: What are the Types of Differential Stains? explain with an example.
A: Bacteria are prokaryotes that are devoid of any membrane-bound organelles. They are differentiated…
Q: What are the possible sources of contamination that may result during the streak plate procedure?
A: The streak plate procedure is used to isolate microbial colonies in pure form. During the procedure,…
Q: Compare and contrast simple, differential, and structural stains, and give examples for each.
A: Introduction Staining Is A Technique For Enhancing Contrast In Specimens, Usually On A Microscopic…
Q: What are the principle and basic concepts of Simple staining
A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…
Q: a. Why must heat be used with the application of the primary stain during endospore staining?
A: Defination- An endospore is a non -reproductive structure which is dormant and tough produced by…
Q: Describe the procedure and purpose for making streak plate.
A: Streak plates involves the streaking of the inoculum over the surface of the agar resulting in its…
Q: Differentiate the “hot method” and “cold method” of acid-fast staining
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise…
A: The preparing a slide that has to be observed under a microscope there are multiple steps that are…
Q: Differentiate between simple and differential stains
A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question…
Q: Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure?
A: Plate counting is one of the most fundamental techniques used in microbiology. It is used to…
Q: Can hand sanitizer kill bacteria?
A: Sanitizer is referred to as any substance or fluid that is used to prevent the spread of pathogens…
Q: Why is it necessary to use contrasting stains while staining
A: Introduction:- Cell staining is a technique that allows you to see cells and cell components more…
Q: What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain,…
A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…
Q: The acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C.…
A: Acid fast bacteria are gram positive bacteria . They are unique among prokaryotes and normal gram…
Q: Positive Staining Negative staining
A: Staining Procedure Dye Charge Of The Chromophore/ Colored Ion Charge Of The Target Cellular…
Q: The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C.…
A: To differentiate between the vegetative cells and the endospores, a differential staining technique…
Q: Why do we need to stain microorganisms?
A: Microbiology is the study of microbes. Microorganisms include bacteria, fungus, archaea, and…
Q: What is the best streaking technique in a culture media? Explain
A: In microbiology, there are five I's that symbolise five different procedures used in the laboratory…
Q: Since the dyes for the Gram stain are not specific to the bacteria theystain, what mistakes in the…
A: Gram staining is a technique used for staining the microbial samples. It was performed by the…
Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?
A: Introduction We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…
Q: Why should freshly collected samples be used for microscopic examination?
A: The benefit of the microscope is that it produces a better resolution than the naked eye. The higher…
Q: How is staining techniques useful in the clinical settings
A: Staining: A sample at the microscopic level can easily be visualized and distinguished from the…
Q: For What purpose is Giemsa stain used for?
A: Staining is a technique used in microscopy. Staining is mainly used in biology to highlight the…
Q: stain
A: Cartilage is a smooth elastic tissue, it protects the bones at the joint by padding it.
Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
Q: cold method of Acid-fast staining.
A:
Q: Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation…
A: Flagella is a hair-like structure that is found in bacteria, archaea, and Eukaryota. It allows the…
Q: What is the purpose of using the streak plate method?
A: Solution- Introduction - Streak plate technique is used for the isolation into a pure culture of…
How can the thickness of the smear affect the staining procedure?
Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells are too small to be observe clearly staining is done to enhance its visualization, and to make the cellular organelles visible under the microscope.
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- Why are basic dyes more effective for bacterial staining than acidic dyes?Jessica placed crystal violet ,grama's iodine and safranin reagents on her bench.Which staining procedure is she preparing for with these reagents? a)Grama's staining procedure b)simple staining procedure c)Acid -fast staining procedure d)Non -acid fast staining procedure e)Scaeffer -Fulton staining procedureHow will the staining appear if if the destaining step were done at high temperature?
- During staining, the first step is to dewax the slide. What is the purpose of the step? What is the effect of insufficient dewaxing?Explain the mechanism of staining for each of the stains used. Iodine Methyl green NigrosinWhy are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation?