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- What is a screening test? Immunoassay Enzyme immunoassay (EAI) Enzyme-multiplied immunoassay technique (EMIT) Fluorescence polarization Radioimmunoassay (RIA) Chromatography Thin-layer chromatography (TLC) Gas chromatography (GC) Liquid chromatography (LC) (i.e, high performance liquid chromatography or HPLC) What is a confirmatory test? Hyphenated technique. Combination of two sophisticated technologies (I.e., Gas Chromatography - mass spectrometry or GC-MS) or other modern and acceptable techniques (l.e., LC-MS, GC-MS-MS, or LC-MS-MS).https://youtu.be/w7aIxiZQ60g Multiplexing agglutination https://youtu.be/uWStmyJ5Qc0 This is the multiplexing agglutination. Lab report I don’t really know what to talk about, the data, conclusions and the purpose of this. Need help pleaseSerial dilutions are made in order to determine the level of the antibody in the sample. True or False ?
- Counterstaining with Hematoxylin and Eosin is an important step in which of the following techniques? ELISA Gel Electrophoresis IHC Western blottingWhich portion of the blood is used for the Elisa test? Explain.Wil completed a full plate titer assay and there were 25 plaques on his 10^-7 plate. What is the titer of his high titer lysate? 10uL of each dilution were used for the plaque assays. O 2.5X10^11 pfu/mL O 2.5X10^7pfu/mL O 2.5X10^9 pfu/mL O 2.5X10^8 pfu/mL O 2.5X10^10 pfu/mL
- Why does the antibody titer determination use twofold dilutions ofthe antiserum rather than 10-fold dilutions?Define Blue/White Screening. Explain principle mechanism of this approach briefly.What are the ordered steps of an ELISA protocol? A. Add primary antibody->wash-> Bind sample to a surface ->Add substrate ->Add secondary antibody-enzyme conjugate ->wash B. Bind sample to support -> Add substrate -> Add primary antibody -> wash -> Add secondary antibody-enzyme conjugate -> wash C. Bind sample to a surface -> Add primary antibody -> wash -> Add secondary antibody-enzyme conjugate -> wash -> Add substrate D. Add secondary antibody-enzyme conjugate -> wash -> Add primary antibody -> wash -> Add substrate -> Bind sample to surface
- Give five (5) other examples of samples that are best prepared using Smear Preparation Technique (example: Human RBCs). Explain why.QUESTION 2 How does Complement fixation test differ from haemolytic titration assay for complement? (Answer should not be more than three lines). QUESTION 3 ELISA technique was used to assay all three pathways of complement. Which molecule will be adsorbed to the ELISA plate in each of the pathways. QUESTION 4 How can radial immunodiffusion be used to demonstrate complement function assay? (Answer should not be more than two lines) QUESTION 5 A 3-year-old child has a history of serious infections and is currently hospitalized with meningitis. The doctor suspects that he may have a complement deficiency and orders testing. A buffer that chelates calcium was added to specimen. There was a decrease radial haemolysis on agarose plate a) Which complement pathway accounted for the result obtained and how will the result in terms of hemolysis at 50 %? b) What is hydrodynamic focusing and why is it an important feature of an instrument.You are tasked with measuring the quantity of prostate specific antigen (PSA) in a urine sample using an ELISA strategy. Describe and schematically depict how you would perform the test given the following materials: anti-PSA, biotinylated anti-PSA, streptavidin-HRP, HRP substrate kit (HRP = horse radish peroxidase).