Q: Is it important to have fewer phages than bacterial host cells when doing a quantitative plaque…
A: A virus is an infective agent, which is sub-microscopic in nature. It has the capability of…
Q: What is the difference between an attenuated and inactivated vaccine?
A: Any foreign substance invading the body and having the capability of stimulating the immune response…
Q: If you wanted to make an assay detecting SARS-CoV-2 virus presence, like the assay shown in the…
A: Enzyme-linked immunosorbent assay (ELISA) is a disease detection method (AIDS) and it works on the…
Q: Why is serotyping particularly important in Salmonella infections and typhoid fever
A: The serotypes are the groups within a single species of microorganisms which may be bacteria or…
Q: What is the etymology (origin) of the word "vaccine"?
A: Answer- The "vaccine" word drived from latin Word VACCA mean cow According to World health…
Q: How we can Treatment Severe Combined Immunodeficiency (SCID) by using gene therapy? Please answer…
A: SEVERE COMBINED IMMUNODEFICIENCY:- SCID stands for severe combined immunodeficiency, a group of…
Q: Why is the ELISA test used to screen human serum for the AIDSvirus, while the Western blot procedure…
A: Answer: Introduction: The human immunodeficiency viruses (HIV) are the type of a subgroup of…
Q: Explain the statistical findings regarding the lateral flowimmunochromatographic assays sensitivity…
A: The lateral flow test is one of a simpler immunochromatographic method that confirms the absence or…
Q: Explain the basic methods behind the Western blot andcomplement fixation tests.
A: Western blot is a specific test that is used to detect the presence of a target protein in the given…
Q: In the SARS-CoV-2 assay, what is the benefit of a diagnostic tool that can distinguish the…
A: The novel Coronavirus infection SARS CoV 2 can be diagnosed by different means. Proper diagnosis,…
Q: What technique could be used to confirm the presence or absence of plasmids in the donor, recipient…
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Define the Genetic Analysis for Pathogen ?
A: Introduction: A pathogen is basically any organism that feeds, inhabits, and reproduces within its…
Q: What is the name of protein subjected to IP? What is the importance of the pY blot? How many…
A: Immunoprecipitation or IP is a technology that precipitates a protein from the solution with the…
Q: EIAs are extremely sensitive diagnostic tools. Why,then, is the immunoblot (Western blot) procedure…
A: EIA stands for enzyme immunoassay, is a diagnostic test that can be divided into two types based on…
Q: What is DNA barcoding, and what types of cases use this profiling method?
A: DNA barcoding is an instrument to recognize various species. Mitochondrial DNA inheritance ( mtDNA)…
Q: Discuss the key events in the production of recombinant subunit vaccine?
A: The technology used for joining different DNA molecules from different species is known as…
Q: how does teh ELISA help determined who the initial disease carrier is.
A: The enzyme linked immunosorbent assay is an analytical biochemistry assay which uses a solid phase…
Q: Why is it important to use the HTAC recommendation framework with the FDA directive towards the…
A: The SARS-CoV-2 virus causes Coronavirus Disease (COVID-19), an infectious disease. The majority of…
Q: How do you use CRISPR to treat a patient with sickle cell anemia? Please provide a detailed…
A: In sickle cell anemia the blood cells become sickle shaped. This may result into painful blood flow,…
Q: What is a recombinant DNA vaccine? List two such vaccines. State their advantages.
A: A vaccine is a preparation of killed or weakened or a part of microbe (antigen) that is used to…
Q: How does plating efficiency affect the number of plaque-forming units? How is plating efficiency…
A: A viral plaque is defined as the visible structure formed on the culture plate of bacteria or any…
Q: I understand that microarrays are being used to define the molecular abnormality and the prognosis…
A: Leukemia and types
Q: why do we have to do a western blot confirmatory testing after doing an ELISA screening test for…
A: The human immunodeficiency virus causes the chronic virus infection known as acquired…
Q: What are advantages and disadvantages of the antibody test with the coronavirus?
A: We know that The Severe Acute Respiratory Syndrome corona virus-2 (SARS-CoV-2) is responsible for…
Q: What is the principle of :Density gradient centrifugation technique that commonly used for the…
A: For mononuclear cell isolation, the density gradient centrifugation is performed
Q: What is the purpose of combining capsule polysaccharide molecules with toxoid proteins in a…
A: A conjugate vaccine is a substance that is composed of a polysaccharide antigen fused (conjugated)…
Q: What are the main procedures involved in a western blot?
A: BASIC INFORMATION WESTERN BLOT This process is used to find some specific type of proteins which are…
Q: state the principle and the general procedure behind indirect serologic testing.
A: Serological tests are used for diagnosis or surveillance purposes. They can determine and indicate…
Q: What are steps in making an inactivated vaccine?
A: Vaccinations and Immunizations started in the 18th century, with the Smallpox Vaccination, created…
Q: How is signal amplification achieved in enzyme-linked immunosorbent assays?
A: Enzyme-linked immunosorbent assays or ELISA refers to the techniques to detect antibodies, hormones,…
Q: What is the purpose of using Triton X in hemolytic assay.
A: Hemolysis is release of hemoglobin into the blood after the red blood cells are lysed. In hemolytic…
Q: Bacterial mutants can be classified on the basis of the method, Which is those methods?
A: Microorganisms undergo mutations under environmental stress that causes sudden heritable changes in…
Q: What are the important differences among a recombinant liveattenuated vaccine, a vector vaccine, and…
A: Vaccination is the process of inoculating an individual with antigenic particles, which can elicit…
Q: The purpose of the QVIR strategy is to
A: Introduction QVIR falls under a class of antiviral drugs known as reverse transcriptase inhibitor
Q: In the plaque assay, what is the precise origin of a single plaque?
A: Answer: Introduction: Virus is an intracellular infectious parasite consist of only one type of…
Q: Which is more proper sample type for whole genome sequencing in AML patients? Peripheral blood or…
A: On the basis of ELN recommendations, one can use PBC samples to diagnose AML if blast count >…
Q: What is a recombinant DNA vaccine?Give two examples.
A: Vaccines are either attenuated or dead agents of disease which when administered into a healthy…
Q: In the plaque assay, exactly what makes up a single plaque?
A: Viruses are non-cellular pathogenic particles. Viruses cannot survive on their own as they lack cell…
Q: explain or describe the principle of immunoturbidimetric C3 Assay.
A: Immunoturbidimetric: A technique that calculates the absorbance of light from an example which is…
Q: What is the advantage of a DNA vaccine over all the other types of vaccine ?
A: DNA vaccination has shown significant promise since its start. In terms of effectiveness, safety,…
Q: What amino acid does the Bradford Protein Assay primarily mesure
A: Introduction: The Bradford assay is a simple and relatively sensitive method for measuring protein…
Q: What methods are used to identify the serovar when there is a Salmonella outbreak? What other…
A: Serovars can be defined as different strains or variants of a bacterial species or a virus that have…
Q: What does BCR ABL positive mean?
A: The cancer of the white blood cells is known as Chronic myelogenous leukemia (CML) in medical terms.…
Q: What is HIV? Discuss what an immunochromatographic assay is If a positive band appears in the test…
A: The Human Immunodeficiency Virus (HIV) is a virus that causes infection in humans. It is a virus…
Q: How reliable are Lateral flowimmunochromatographic assays when compared to molecular diagnostic…
A: COVID-19 is a respiratory disorder caused by a novel coronavirus (SARS-CoV-2). The genetic…
Q: What is the difference between the total and faecal coliform
A: MPN assay is the statistical, multi-stage test for determining the presence of bacteria in water,…
What is rationale of using serological typing in bacterial agglutination?
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- What are the advantages and disadvantages of Nasopharyngeal Swab Test through Reverse-Transcriptase Polymerase Chain Reaction?Why is the ELISA test used to screen human serum for the AIDSvirus, while the Western blot procedure is used only as the confirmation test?what are some advantages to using latex particles instead of blood cells for IM assay?
- why do we have to do a western blot confirmatory testing after doing an ELISA screening test for HIV?One laboratory method to detect for COVID-19 infection is via real time RT-PCR. The process starts with nasopharyngeal or oropharyngeal swabbing to get a sample. Explain concisely how this technique generally works and how it can be used for testing.What is the recommended method to identify an antibody causing a suspected ABO based HDN( Hemolytic Disease of newborn)?
- How reliable are Lateral flowimmunochromatographic assays when compared to molecular diagnostic tests or other serological tests? Please discuss referring to original articles example of covid 19..How is RT PCR used to detect Ebola? How does the actual RT PCR procedure work? How is ELISA used to detect Ebola? What is the method? What binds to what? How is Immunohistochemistry (IHC) used to detect Ebola? What is the method? What tissue is used, what binds to what?Why is serotyping particularly important in Salmonella infections and typhoid fever?
- what is the principle of ELISA? What is the procedure of direct and indirect ELISA and what is the purpose of each variant in clinical diagnosis?Briefly describe multi locus sequence typing (MLST) as it relates to the identification of Listeria monocytogenes.What is the purpose of using Triton X in hemolytic assay.