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- 1) What are you testing in the picture above? 2) How would you interpret this test? 3) What is the basis for this type of test?why do we have to do a western blot confirmatory testing after doing an ELISA screening test for HIV?What is the point of doing a modified Furter-Meyer Test? What is the underlying idea of this test? How did you figure out that this test had a positive result?
- What are the advantages and disadvantages of Nasopharyngeal Swab Test through Reverse-Transcriptase Polymerase Chain Reaction?What is a recombinant DNA vaccine? List two such vaccines. State their advantages.Why is the ELISA test used to screen human serum for the AIDSvirus, while the Western blot procedure is used only as the confirmation test?
- Many ELISAs that are used to identify the presence of antibody to viruses have a 3rd control (along with negative and positive). In between the rows of wells coated with antigen, are rows of cells coated with tissue culture used to grow the virus. In other words, patient serum is added to wells that contain antigen along with wells that contain tissue culture. Why do you think this might be necessary?What is the purpose of conducting a modified Furter-Meyer Test? What is the principle behind this test? How could you identify the positive result for this test?EIAs are extremely sensitive diagnostic tools. Why,then, is the immunoblot (Western blot) procedure usedto confirm screening tests that are positive for humanimmunodeficiency virus (HIV)?
- What is a recombinant DNA vaccine?Give two examples.What are the advantages and disadvantages of agglutinationtests versus fluorescent antibody assays? How are the latter usedto identify specific cells in complex mixtures, such as blood?Explain how PCR/OLA (polymerase chain reaction/oligonucleotide ligation assay) can be used in the diagnosis of sickle cell disorder . Would you recommend this method for routine diagnosis of sickle cell disorder? Explain