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- mead. Post Lab #2 Smear & Stain Preparation Name: Leidiawa MontaNo Date: 1. Why are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation? Please explain. Becapse, it will diminish the amount ds latcan Pass through by mam 1t difficult to See under the micioscol e, s less liket to Prowde a go image. 2. What could potentially happen if you leave the slide exposed for too long to the open flame? Why do you have to be careful? we can form ring Patterns if we expose the slide for ta0 the flame 3. During the preparation of a smear leading into simple staining of the bacterial culture S. epidermidis you forgot to heat fix the slide. What would you see on this slide as compared to a slide that was properly prepared? Please explain. 4. You partner stained bacterial cells and saw only the background and not the actual cell was stained. Your partner thought this was a mistake. Please explain what type of staining method this is, how it works and why the…blackboardcdn.com/5bfc08ba3fldc/14683296?X-Blackboard-Expiration=16245468000008X-Blackboard- 19 / 47 100% 1.4. Functions of the light mlcroscope parts Complete the following table by writing the function(s) of each of the parts indicated. Structure Function Diaphragm / iris Stage opehing Lamp Objective lenses Eye piece Coarse and find adjustment knobs Stage Stage rack prt sc delete home backspace lock enter pause t shiftDescribe your results in the following data chart as directed in the instructions for the laboratory exercise. Treatment Control 0.01% Bleach 0.1% Bleach 1.0% Bleach 2.5% Lysol 5% Lysol 10% Lysol 0.03% Hydrogen Peroxide 0.3% Hydrogen Peroxide 3.0% Hydrogen Peroxide 10% Isopropyl Alcohol 30% Isopropyl Alcohol 50% Isopropyl Alcohol Growth
- Immediately after stirring 1 hour after stiring Original misture Suction filiration Figure 1 Figure 2 Figure 3 Various tests are performed on the mixtures as shown in the figures above. Identify any correct explanations of these tests below. [Check all statements that are true.] In figure 1, a laser is passed through two tubes of clear liquid. The liquid in the tube on the left is a solution, while the liquid in the right tube is not a solution. O In figure 2, a recently stirred mixture is allowed to stand for one hour. The original mixture is a solution while the mixture after 1 hour is not a solution. In figure 3. a mixture is filtered using suction filtration. A solid remains on the filter. The original mixture was a solution.The significance of using immersion oil when using 1OOX objective lens is The significance of using immersion oll when uskng 100X objective lens is: It will increase the contrast by having same refractive index as the lens, loss of light by refraction, reflection and diffraction is minimized. the specimens need not be killed, fixed and stained more light is captured for better illumination, allowing the specimen to fluoresce i. il. iii. iv. A light microscope should have the following features contrast Resolution magnification fluorescence ii. iv.Make a concept map/flow chart for this technique (Cellulose Tape Perianal Swab)
- My field is COSMETIC SCIENCE What information can be obtained from UV-vis Spectra and how this can be applied in your chosen field? (maximum of 500 words)Condition; Acne Prone Skin Can we only use the facial gel A (facial gel A compose of water, Polysorbate 80, Propylene Glycol, Glycerin, Aloe Barbadensis Leaf Juice Powder, Carbomer, Hydroxypropyl Methylcellulose) wash the face to rinse off the sunscreen and any skincare in form of cream or serum, and does it enough if we use micellar water to clean the face first and then wash the face by using facial gel A for water-resistant sunsceen.34. A dermatologic lotion contains 1.25 mL of liquefied phenol in 500 mL. Calculate the percentage (v/v) of liquefied phenol in the lotion. 34. 0.25% v/v liquefied phenol
- 8. The ___________ color test reagent turns orange-brown in the presence of amphetamines.?Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possibleChemical tests See bottom of page 8 for instructions on how to complete this table. C. Biuret test Solution turins olive greeni Yeu ight Pur ple A. Iodine test B. Benedict's test Description of Solution turne Slohtion turns positive result Tube No. and (copy from Contents dark Purple or brovniornd asbiutrldeins Pp. 7-8) decp blue-blakk or tomoto of Short +/- Appearance blue (from p. 9) らish | +/- | Appearance blae Appearance +/- 1 - Orense 2 - Glucose Starch orunde Orange 3 - ders Brtelt blue blue blve bue 4 - alanine oransC- blue - 5. blue iout bue t orange Why was it important to have tube number 1 in each test? (Don't just say "because it's the control", and don't copy from the lab instructions-write out your own understanding of why and how that tube was used.)