mead. Post Lab #2 Smear & Stain Preparation Name: Leidiawa MontaNo Date: 1. Why are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation? Please explain. Becapse, it will diminish the amount ds latcan Pass through by mam 1t difficult to See under the micioscol e, s less liket to Prowde a go image. 2. What could potentially happen if you leave the slide exposed for too long to the open flame? Why do you have to be careful? we can form ring Patterns if we expose the slide for ta0 the flame 3. During the preparation of a smear leading into simple staining of the bacterial culture S. epidermidis you forgot to heat fix the slide. What would you see on this slide as compared to a slide that was properly prepared? Please explain. 4. You partner stained bacterial cells and saw only the background and not the actual cell was stained. Your partner thought this was a mistake. Please explain what type of staining method this is, how it works and why the background and cell is not stained. 5. Take a look at your streak plate from the use of a mix culture. Does your plate show the results that are expected? If yes, what does this result tell you if no, what error do you think could've caused this? Explain. 6. You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.
mead. Post Lab #2 Smear & Stain Preparation Name: Leidiawa MontaNo Date: 1. Why are thick or dense smears less likely to provide a good smear preparation for microscopic evaluation? Please explain. Becapse, it will diminish the amount ds latcan Pass through by mam 1t difficult to See under the micioscol e, s less liket to Prowde a go image. 2. What could potentially happen if you leave the slide exposed for too long to the open flame? Why do you have to be careful? we can form ring Patterns if we expose the slide for ta0 the flame 3. During the preparation of a smear leading into simple staining of the bacterial culture S. epidermidis you forgot to heat fix the slide. What would you see on this slide as compared to a slide that was properly prepared? Please explain. 4. You partner stained bacterial cells and saw only the background and not the actual cell was stained. Your partner thought this was a mistake. Please explain what type of staining method this is, how it works and why the background and cell is not stained. 5. Take a look at your streak plate from the use of a mix culture. Does your plate show the results that are expected? If yes, what does this result tell you if no, what error do you think could've caused this? Explain. 6. You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.
Chapter12: Parenteral Equipment And Supplies
Section: Chapter Questions
Problem 25RQ
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Transcribed Image Text:mead.
Post Lab #2
Smear & Stain Preparation
Name: Leidiawa MontaNo
Date:
1. Why are thick or dense smears less likely to provide a good smear preparation for
microscopic evaluation? Please explain.
Becapse, it will diminish the amount ds latcan Pass through by mam
1t difficult to See under the micioscol e, s less liket to Prowde a go
image.
2. What could potentially happen if you leave the slide exposed for too long to the open
flame? Why do you have to be careful?
we can form ring Patterns if we expose the slide for ta0
the flame
3. During the preparation of a smear leading into simple staining of the bacterial culture
S. epidermidis you forgot to heat fix the slide. What would you see on this slide as
compared to a slide that was properly prepared? Please explain.
4. You partner stained bacterial cells and saw only the background and not the actual cell
was stained. Your partner thought this was a mistake. Please explain what type of
staining method this is, how it works and why the background and cell is not stained.
5. Take a look at your streak plate from the use of a mix culture. Does your plate show
the results that are expected? If yes, what does this result tell you if no, what error do you
think could've caused this? Explain.
6. You receive a bacterial culture in the lab and your job is to figure out information
which will be helpful. You are only allowed to use one of the staining methods we
practiced. Which one would be the best option? Why? And what will this information
help you determine? Explain.
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