What allows for intervening sequence between a promoter and a regulatory to be looped out so that a regulatory protein can contact RNA polymerase?
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- What would be the likely effect of a mutation that prevents s from dissociating from the RNA polymerase core?a) what is a promoter and give the element and their functions of E.coli promoter b) what are eukaryotic transcription factor and list the class 2 general transcription factors and state their functionsWhy is the RNA polymerase/RNA/DNA duplex of the RNA polymerizing complex called transcription bubble? What is the local change in Lk, Tw and Wr within the RNA polymerase DNA transcription bubble?
- How do we know that core-promoter elements are importantfor transcription?Please describe why regulationof transcription frequently involves the promoter and protein interactions with the promoter. Please add details about bacterial promoter properties to discuss RNA polymerase binding to initiate transcription.How does the 4 feature of transcription factors namely the structural motifs of DNA binding protein, activation domains, multiple transcription factors and enhancers help in the design of a building block tool. U can use the SrY gene as ur building block tool. Pls explain in details using those features of the transcription factors. In 400 words
- When an activator protein interacts with mediator, how does this affect the function of RNA polymerase?What is the production of RNA called and what is the enzyme that catalyzes the process?What are the similarities and differences between the transcription process and the repli-cation processes?Concerning their biological function what is the difference between DNA and RNA? Is there any situation in which DNA is made based on a RNA template? If there is,explain with an example how it occurs and state the enzyme involved?What is the difference between plasma membrane and cell wall?The method of Northern blotting is used to determine the amountand size of a particular RNA transcribed in a given cell type.Alternative splicing (discussed in Chapter 14) produces mRNAsof different lengths from the same gene. The Northern blot shownhere was obtained using a DNA probe that is complementary tothe mRNA encoded by a particular gene. The mRNA in lanes 1through 4 was isolated from different cell types, and equal amountsof total cellular mRNA were added to each lane. Explain these results.
- Using the transcription unit diagrammed below, in which exons are represented by blue boxes and introns are represented by the connecting lines. You discover a single base deletion in region E of this DNA sequence. Regarding transcription, this mutation will likely: 1.) Result in an alteration to the mRNA sequence. 2.)Have no effect on transcription or the mRNA sequence 3.)Prevent transcription at the TATAA box 4.) Result in an increase or decrease in the amount of mRNA transcribedSome prion-related diseases, such as familial fatal insomnia, areinherited. How would you expect the mutation has altered the PrPgene in this case? Would it have affected the promoter, the regulatorysequences, or the coding sequence of the PrP gene?Microbiologists describe the processes of transcription and translation as “coupled” in bacteria. This term indicates that bacterial mRNA can be undergoing transcription at the same moment it is also undergoing translation. How is coupling possible in bacteria? Is coupling of transcription and translation possible in single-celled eukaryotes, such as yeast? Why or why not?