2) Assume that you made an error during your staining procedure. You used safranin as the primary stain and malachite green as counterstain. How would your cells appear at the end of the staining of the staining technique?

2) Assume that you made an error during your staining procedure. You used safranin as the primary stain and malachite green as counterstain. How would your cells appear at the end of the staining of the staining technique?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.7: Preparing And Staining A Blood Smear

Problem 1.2CS

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Concept explainers

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.

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2) Assume that you made an error during your staining procedure. You used safranin as the
primary stain and malachite green as counterstain. How would your cells appear at the end of
the staining of the staining technique?

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In four steps, describe the simple staining procedure.
Select all that apply to a negative stain: 1. involves a washing step 2. cells may be distorted or shrunken 3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cells
What are the principle and basic concepts of Simple staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)
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How will the staining appear if if the destaining step were done at high temperature?
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