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How will the staining appear if if the destaining step were done at high temperature?
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- Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can confirm whether a bacterial smear has been correctly prepared or not. Why should you be careful not to underheat a smear during the heat-fixing process? Why do you think the presence of grease or dirt on a glass slide will result in a poor smear preparation? Cite two or three reasons.Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?
- Describe the Gram staining procedureIn preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?Identify that chemical mordant used in the cold method of Acid-fast staining.
- What would happen if you had skipped the heat fixing step and continued with the staining procedure?What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.During staining, the first step is to dewax the slide. What is the purpose of the step? What is the effect of insufficient dewaxing?