Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Chapter 4.2, Problem 2TYK
Summary Introduction
Introduction:
The technique of microscopy helps to identify micro-organisms and visualize various physiological features of the organism under study. Different types of microscopes are used for various purposes that allow easy detection and exhaustive study of the structure and properties of microbes.
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Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?
Which of the following statements are true in the context of the microscope's field of view?
I. It is the observable area within a circular space as defined by the lenses
II. FOV is the smallest on the lowest power objective
III. FOV is the maximum area visible through the microscope eyepiece
IV. When you switch to a higher power, the field of zooms out towards the center
Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.
Chapter 4 Solutions
Principles of Biology
Ch. 4.1 - With regard to the origin of life, why are...Ch. 4.1 - Prob. 2CCCh. 4.1 - Prob. 3CCCh. 4.1 - Prob. 1BCCh. 4.1 - Prob. 4CCCh. 4.1 - Prob. 1TYKCh. 4.1 - Prob. 2TYKCh. 4.1 - Prob. 3TYKCh. 4.2 - Prob. 1CCCh. 4.2 - Prob. 1TYK
Ch. 4.2 - Prob. 2CCCh. 4.2 - Prob. 2TYKCh. 4.3 - Prob. 1BCCh. 4.3 - Prob. 1CCCh. 4.3 - Prob. 2BCCh. 4.3 - Prob. 1TYKCh. 4.3 - Prob. 2TYKCh. 4.3 - Prob. 3TYKCh. 4.4 - Prob. 1BCCh. 4.4 - Prob. 1CCCh. 4.4 - Prob. 1TYKCh. 4.4 - Prob. 2BCCh. 4.4 - Prob. 2TYKCh. 4.4 - Prob. 3TYKCh. 4.5 - What is the function of the nuclear lamina and the...Ch. 4.5 - Prob. 1BCCh. 4.5 - Prob. 2CCCh. 4.5 - Prob. 1TYKCh. 4.5 - Prob. 2TYKCh. 4.5 - Prob. 3TYKCh. 4.6 - What is the advantage of having a highly...Ch. 4.6 - Prob. 1BCCh. 4.6 - Prob. 2CCCh. 4.6 - Prob. 1TYKCh. 4.6 - Prob. 2TYKCh. 4.7 - Prob. 1TYKCh. 4.8 - Prob. 1CCCh. 4.8 - Prob. 2CCCh. 4.8 - Prob. 3CCCh. 4.8 - Prob. 4CCCh. 4.8 - Prob. 1TYKCh. 4.8 - Prob. 2TYKCh. 4.8 - Prob. 3TYKCh. 4.9 - Which of the following could be found in a...Ch. 4.9 - Prob. 2TYKCh. 4 - The cell theory states that all living things are...Ch. 4 - Prob. 2TYCh. 4 - Prob. 3TYCh. 4 - Prob. 4TYCh. 4 - Prob. 5TYCh. 4 - Prob. 6TYCh. 4 - Prob. 7TYCh. 4 - Prob. 8TYCh. 4 - Prob. 9TYCh. 4 - Prob. 10TYCh. 4 - Describe two specific ways that protein-protein...Ch. 4 - Explain how motor proteins and cytoskeletal...Ch. 4 - A principle of biology is that structure...Ch. 4 - Prob. 1CBQCh. 4 - Prob. 2CBQ
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- In light microscopy, when magnification is increased, the lens focuses closer to the sample. This makes the user more likely to ram the sample into the lens. Why is it common for the lens to focus closer to the sample at higher magnifications? What can be done to mitigate this risk?arrow_forwardWhich of the following microscopes produce flat images by utilizing light in the non-visual spectrum to excite molecules within the specimens to produce visual light? (select all answers that apply) Phase Contrast Microscope Darkfield Microscope Fluorescent Microscope Brightfield Microscope Electron Microscope Confocal Microscopearrow_forwardDifferentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…arrow_forward
- Total magnification of a microscope is determined by Multiple Choice dividing the objective magnification value by the ocular value. multiplying all objective magnification values together. adding all objective magnification values together. none of the above.arrow_forwardOf the four major types of microscopes, give an example of a scenario in which each would be the ideal choice for visualizing a sample. Stereo (Dissecting) Compound Confocal Electronarrow_forwardWhich of the following statements regarding electron microscopy is FALSE? Question options: live organisms cannot be viewed with an electron microscope all of these statements are TRUE scanning electron microscopes focus electrons across the surface of a sample electrons pass through a sample viewed using transmission electron microscopy electron microscopes use magnetic fields in place of glass lenses electron microsopes have a better resolving power than light microscopesarrow_forward
- Which of the following is a parameter of microscopy that can affect your ability to resolve a specimen using a compound light microscope? Question options: Contrast Resolving power All of the choices Numerical aperturearrow_forwardGiven: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Questions: What is the total magnification of the image under microscope? Show your solution. What is the distance of one ocular division? Show your solution. What is approximate cell length of Cell A, Cell B, and Cell C? What is approximate combine cell length of Cell A and Cell B? Show your solution. Remember: Always measure the cell length on the longest middle section of the cell!arrow_forwardGiven: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the total magnification of the image under microscope? Show your solution.arrow_forward
- Which of the following microscopy techniques can be used in live cell imaging in a live animal. 1. Structured illumination microscopy 2. Stimulated emission depletion microscopy 3. Photoactivated localization microscopy 4. Two-photon excitation microscopy 5. Total internal reflection microscopy 6. Light-sheet microscopy O4 and 6 O2, 3 and 6 O1 and 4 3 and 5 O2 and 4arrow_forwardPrevious question: For example, you can estimate the field of view diameter at 400x total magnification using the measured diameter at 100x total magnification. The magnification is 4 times greater so the field of view will be 4 times smaller. What is the field of view under the 40X objective of the microscope. - The Answer I got for this question was 3/4mm.arrow_forwardWhat information can be obtained from the slides prepared by wet mount and hanging drop technique for microscopic observation? Give at least three (3) information. What are the limitations of each method. (Provide 2 limitations)arrow_forward
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