Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Textbook Question
Chapter 28.3, Problem 1CR
Q Why is it important to process clinical specimens as rapidly as possible? What special procedures and precautions are necessary for the isolation and culture of anaerobes?
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Briefly describe the main scientific principle(s) underlying the following techniques used in the Combating Disease practical (you may use diagrams if you wish):
ammonium sulphate precipitation
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困
Topic: laboratory Instrument
Which of the following are TRUE regarding
Which of the following is/are TRUE
the use of laboratory instruments and
regarding the use laboratory instruments
equipment in regards to making of culture
media? "
and equipment? *
Air bubbles on the surface of agar plates
Hot plates can be used to sterilize the
inoculating loop and needle.
can be removed by fanning them with the
luminous flame of the bunsen burner.
If the agar surface is wet, it can be dried by
A drying oven is used to sterilize nutrient
agar, a solid culture medium.
heating at 30-40 deg C in the drying oven
before inoculation is done.
The refrigerator is used to grow
psychrophiles.
All media taken from the refrigerator should
be warmed to room temperature before
use,
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Chapter 28 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 28.1 - The use of personal protective equipment (PPE) is...Ch. 28.1 - Identify and discuss the standard safety...Ch. 28.1 - Prob. 1CRCh. 28.2 - Prob. 1MQCh. 28.2 - How can the spread of HAIs be controlled?Ch. 28.2 - Prob. 1CRCh. 28.3 - What are the key points necessary for proper...Ch. 28.3 - Identify culture methods and conditions used for...Ch. 28.3 - QWhy is it important to process clinical specimens...Ch. 28.4 - Describe the disc diffusion test and the Etest for...
Ch. 28.4 - What is the value of antimicrobial drug...Ch. 28.4 - QDescribe the disc diffusion test for antibiotic...Ch. 28.5 - Explain the reasons for changes in antibody titer...Ch. 28.5 - Describe the method, time frame, and rationale for...Ch. 28.5 - What advantages do monoclonal antibodies have...Ch. 28.5 - QWhy does antibody titer rise after infection? Is...Ch. 28.6 - How is the bivalence of antibodies significant for...Ch. 28.6 - What are the advantages and disadvantages of...Ch. 28.6 - Why are agglutination tests so widely used in...Ch. 28.7 - Prob. 1MQCh. 28.7 - Compare the advantages and disadvantages of EIA,...Ch. 28.7 - Prob. 1CRCh. 28.8 - What advantage(s) does nucleic acid amplification...Ch. 28.8 - How do quantitative PCR (qPCR) and qualitative PCR...Ch. 28.8 - Distinguish between quantitative and qualitative...Ch. 28.9 - Compare and contrast live attenuated vaccines,...Ch. 28.9 - Identify the advantages of alternative...Ch. 28.9 - QList the immunizations recommended for children...Ch. 28.10 - Prob. 1MQCh. 28.10 - How does the activity of each antibiotic class...Ch. 28.10 - What are the sources of aminoglycosides,...Ch. 28.10 - Antibiotics are chemically diverse antimicrobial...Ch. 28.11 - What steps in the viral maturation process are...Ch. 28.11 - Why are there fewer clinically effective...Ch. 28.11 - Why is host toxicity a common problem with...Ch. 28.12 - Identify the basic mechanisms of antibiotic...Ch. 28.12 - What does vancomycin have in common with...Ch. 28.12 - Prob. 3MQCh. 28.12 - What practices contribute to the spread of...Ch. 28 - Define the procedures you would use to isolate and...Ch. 28 - Prob. 2AQCh. 28 - Describe three important reasons why semisynthetic...Ch. 28 - Imagine yourself as a clinical microbiologist with...
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- Explain why is it important to follow proper precautions and use aseptic technique when working with clinical samples.arrow_forwardConsidering microbial identification methods, to which category would the following method belong based on what it measures? * Performing a hydrogen peroxide breakdown assay to see if a microbe produces the enzyme catalase Serological O Biochemical Genotypic Phenotypicarrow_forwardPlastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:arrow_forward
- 5) Aseptic technique refers to A) the microbial inoculum placed into a test tube or onto a Petri plate. B) a series of practices to avoid contamination. C) the autoclave and other sterilizing procedures. D) cleanliness in the laboratory.arrow_forwardWhat is the advantage of using Molecular data compared to plated/tube culture or morphological/biochemical techniques in identifying bacterial species especially for oral microbes.arrow_forwardListen Which of the following methods of microbial control is used most often with surgical equipment? a) autoclave b) sugar and salting c) dessication d) lyophilizationarrow_forward
- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperarrow_forwardOn Aseptic Techniques: What is/are the instances/situations that each method of inoculation is to be used?arrow_forward22. A pure culture refers to a culture that contains multiple types of organisms. a) Trueb) Falsearrow_forward
- Explain some of the problems involved in sterilizing delicate andheat-sensitive instruments such as endoscopes, syringes, needles,catheters, and other instruments.arrow_forward26. Describe the steps and techniques from sampling to identification of a microorganism.arrow_forwardExplain the significance of aseptic culture technique in growing microorganisms.arrow_forward
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