Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 19.4, Problem 1CS
Genetic Properties of Bacteria
Core Skill: Connections Look back at Figures 4.8 and 4.9. How is a nucleoid different from a nucleus found in a eukaryotic cell?
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Question -Esther is completing her PhD project in bacterial distribution in mangroves and theirbiochemical characteristics. She manages to isolate and identify around 80 differentbacterial species from the various mangrove sites. She preserves the isolates by growingthem on a tryptic soy agar supplemented with sea salt and re-streaks them every week. After8 months she notices that some of the isolates have changed their genetic sequence whencompared to the initially identified sequence. Using a diagram explain what could havehappened to the isolates when she re-streaked them repeatedly and recommend a solutionto prevent this from occurring in the future and give explanation why it is moreadvantageous.
Objective: Get a sense of how genomics, the study of the genome in its entirety,needs to think about how to go about its research.
Geonomic DNA is broken up into fragments. The 5’ and 3’ ends of each fragment(a “read”) are sequenced. The sequenced reads are assembled together intocontiguous sequences (“contigs”) based on sequence similarity.
The idea is to sequence enough random fragments so that every nucleotide in thegenome is represented on some read. The number of such fragments needed iscalled the coverage, c.
The coverage c can be calculated by the formula RL/G, where R is the number ofreads sequenced, L is the average length of a read and G is the total length of thegenome. The units of length are bases (b) or base pairs (bp).
Consider a genome whose length is 1000 bp. “Shotgun” sequencing techniquesare applied to the genome, resulting in 20 reads, with an average length of 50 bp.A very important point is that, even though 20 x 50 = 1000, there is no guaranteethat ALL…
Objective: Get a sense of how genomics, the study of the genome in its entirety,needs to think about how to go about its research.
Geonomic DNA is broken up into fragments. The 5’ and 3’ ends of each fragment(a “read”) are sequenced. The sequenced reads are assembled together intocontiguous sequences (“contigs”) based on sequence similarity.
The idea is to sequence enough random fragments so that every nucleotide in thegenome is represented on some read. The number of such fragments needed iscalled the coverage, c.
The coverage c can be calculated by the formula RL/G, where R is the number ofreads sequenced, L is the average length of a read and G is the total length of thegenome. The units of length are bases (b) or base pairs (bp).
Consider a genome whose length is 1000 bp. “Shotgun” sequencing techniquesare applied to the genome, resulting in 20 reads, with an average length of 50 bp.A very important point is that, even though 20 x 50 = 1000, there is no guaranteethat ALL…
Chapter 19 Solutions
Biology
Ch. 19.1 - Prob. 1CSCh. 19.2 - Prob. 1CSCh. 19.2 - Viral Reproductive Cycles Concept Check: From the...Ch. 19.4 - Genetic Properties of Bacteria Core Skill:...Ch. 19.4 - Prob. 1CCCh. 19.4 - Genetic Properties of Bacteria Concept Check:...Ch. 19.4 - Prob. 3CCCh. 19.5 - Prob. 1CCCh. 19.5 - Prob. 1EQCh. 19.5 - Prob. 2EQ
Ch. 19.5 - Gene Transfer Between Bacteria CoreSKILL The gene...Ch. 19.5 - Prob. 2CCCh. 19.5 - Gene Transfer Between Bacteria Core Skill:...Ch. 19.5 - Gene Transfer Between Bacteria Concept Check: Is...Ch. 19 - Prob. 1TYCh. 19 - The characteristics of viral genomes show many...Ch. 19 - During viral infection, attachment is usually...Ch. 19 - Prob. 4TYCh. 19 - Prob. 5TYCh. 19 - Prob. 6TYCh. 19 - Prob. 7TYCh. 19 - Prob. 8TYCh. 19 - Prob. 9TYCh. 19 - Prob. 10TYCh. 19 - How are viruses similar to living cells, and how...Ch. 19 - Prob. 2CQCh. 19 - Prob. 3CQCh. 19 - Prob. 1COQCh. 19 - Prob. 2COQ
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