Campbell Biology
Campbell Biology
12th Edition
ISBN: 9780135188743
Author: Urry
Publisher: PEARSON
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Chapter 17.3, Problem 3CC
Summary Introduction

To determine: The effect on the cells that are treated with an agent which caused the removal of cap from messenger RNAs (mRNAs).

Concept introduction:

After transcription, pre-mRNA is synthesized and further modified before translation in the cytoplasm. This is called the RNA processing and it includes the modification of mRNA ends and RNA splicing. A modified guanine (7-methyl guanine) group is added to the 5′ end of the mRNA chain with the help of the capping enzymes. This is known as the 5′ cap. At the 3′ end of the eukaryotic mRNA chain, adenine groups (poly-A tail) are added by poly (A) polymerase enzyme. This is known as the polyadenylation.

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Q34. mRNA decay (breakdown) can play an important role in controlling protein abundance. Which of the following scenarios correctly describes a relationship between mRNA decay and protein abundance?   A. A decrease in transcription with an increase in the rate of mRNA decay can result in increased protein abundance. B. An increase in transcription with an increase in the rate of mRNA decay can result in no change in protein abundance. C. An increase rate of protein synthesis but failure to form an apoprotein can be explained by a decrease in mRNA decay. D. None of the above
Need help:, The rRNAs are isolated from the large subunit of a bacterial ribosome and separated by density gradient centrifugation. Draw the resulting density gradient and label the bands observed. Which rRNA is longest?
E32. In the technique of DNase I footprinting, the binding of a protein to a region of DNA protects that region from digestion by DNase I by blocking the ability of DNase I to gain access to the DNA. In the DNase I footprinting experiment shown here, a researcher began with a sample of cloned DNA 400 bp in length. This DNA contained a eukaryotic promoter for RNA polymerase II. The assembly of general transcription factors and RNA polymerase II at the core promoter is described in Chapter 12 (see Figure 12.14). For the sample loaded in lane 1, no proteins were added. For the sample loaded in lane 2, the 400-bp fragment was mixed with RNA polymerase II plus TFIID and TFIIB. 2 400 350 250 175 50 Which region of this 400-bp fragment of DNA is bound by RNA polymerase II and TFIID and TFIIB? || III ||| | ||||

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