You have carried out an experiment using the spectrophotometry concept. A solution containing compound X is mixed with reagent 1 and then reagent 2. This mixture produces a blue colour whose absorbance (A) could be read at 550 nm. The results are shown below. If the standard solution (compound X) used have a concentration of 1 mM: 1. Calculate the quantity of the standard compound X in μmoles for each test tube (1-7).
You have carried out an experiment using the spectrophotometry concept. A solution containing compound X is mixed with reagent 1 and then reagent 2. This mixture produces a blue colour whose absorbance (A) could be read at 550 nm. The results are shown below. If the standard solution (compound X) used have a concentration of 1 mM: 1. Calculate the quantity of the standard compound X in μmoles for each test tube (1-7).
Chapter19: Calculating Iv Infusion And Completion Times
Section: Chapter Questions
Problem 23SST
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Question
You have carried out an experiment using the spectrophotometry concept. A solution
containing compound X is mixed with reagent 1 and then reagent 2. This mixture produces a
blue colour whose absorbance (A) could be read at 550 nm. The results are shown below.
If the standard solution (compound X) used have a concentration of 1 mM:
1. Calculate the quantity of the standard compound X in μmoles for each test tube (1-7).
Transcribed Image Text:Test
tube no.
2
3
4
5
6
7
Sample
Y
Standard
solution X
(ml)
0
0.1
0.2
0.4
0.6
0.8
1.0
1.0
Reagent 1 Reagent 2
(ml)
(ml)
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
0.3
3.0
Water (ml)
1.0
0.9
0.8
0.6
0.4
0.2
0.0
0.0
A550 nm
0.0
0.05
0.12
0.24
0.35
0.48
0.59
0.35
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