Why were the absorbance measurements recorded at lambda max (504 nm)? Question 1 options: at lambda max impurities in the sample do not absorb any light Because the allura red dye absorbs red color at 504 nm at lambda max the absorbance is the most sensitive to dye concentration at lambda max the absorbance is the least sensitive to dye concentration

Why were the absorbance measurements recorded at lambda max (504 nm)? Question 1 options: at lambda max impurities in the sample do not absorb any light Because the allura red dye absorbs red color at 504 nm at lambda max the absorbance is the most sensitive to dye concentration at lambda max the absorbance is the least sensitive to dye concentration

Organic Chemistry

8th Edition

ISBN:9781305580350

Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Publisher:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Chapter20: Dienes, Conjugated Systems, And Pericyclic Reactions

Section: Chapter Questions

Problem 20.26P

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Which absorbance spectrum from the previous problem represents the dye solution with the greatest concentration? 1.4 1.2 Curve 3 0.8 0.6 Curve 2 Curve 1 0.4 0.2 0.2 Wavelength (nm) O All three are approximately the same concentration O Curve 3 O Cannot be determined from the information given Curve 2 Curve 1 700 580 Absorbance
5. A sample of a red dye and a sample of a blue dye both have the same concentration. Their Absorbance is measured using the same spectrophotometer. At a particular wavelength, will both samples give the same Absorbance reading? Why or why not?
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Test substance-A has a strong absorbance at 525nm (green light) but low absorbance at 730nm (red light). Substance-B has a very low absorbance of green light, but very high absorbance of red light. From this, it can be determined that... Select one: O a. None of these can be determined from the available data. O b. Substance A is fluórescent, but substance B is not. O c. Substance B contains contaminants. O' d. Substance A and substance B are the same material. e. Substance A and substance B are different materials. Clear my choice
NAME PRELAB QUESTIONS These questions should be answered before coming to lab, and included when you turn in your lab outline and data sheets. 1. The dissolved compound in a sample has an absorbance value of 0.250. How would you expect the absorbance to change if: a. The concentration of the dissolved compound is tripled? b. The concentration of the dissolved compound is halved? 2. If you have a solution of unknown concentration, describe how you could determine the approximate concentration. 0.S a.r 0.1 6.0 0.0
The absorbance of a sample as measured by UV-visible spectrometry is inversely proportional to the concentration of the sample, such that, as the concentration increases, the absorbance decreases. True False#
2. You have two solutions of the same compound that are blue. One is very pale and the other is very intense. Absorbance of each solution was measured with a spectrophotometer at the same wavelength at which the solution absorbs. a) Which solution has the higher absorbance? b) Which is the more concentrated solution?
what do the peaks in a IR spectrum represent?
state if true or false 1. Prior to a fixed point or wavelength reading, spectral scanning can be done to determine the maximum absorbance wavelength of a sample. 2. The purpose of a diffraction grating is the same as that of a prism. 3. In any UV-VIS spectrophotometer, the light is filtered first to a single wavelength or narrow range before it hits the sample 4. In any UV-VIS spectrophotometer setup, the detector always comes right after the sample container. 5. It is necessary to set the blank before each reading even if the only difference between the sample solutions is the amount of analyte. 6. Prior to a fixed point/wavelength reading, spectral scanning can be done to determine the maximum absorbance wavelength of a sample.
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Interpret the IR spectrum.
What does the absorbance at 260 nm tells you? How about the 260/280 ratio?

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	at lambda max impurities in the sample do not absorb any light
	Because the allura red dye absorbs red color at 504 nm
	at lambda max the absorbance is the most sensitive to dye concentration
	at lambda max the absorbance is the least sensitive to dye concentration