When we perform Bradford Assay to measure protein concentration, the spectrophotometer is set 595 nm because: a. the excitation peak of Coomassie Blue is best observed at 595 nm b. when Coomassie Blue binds to proteins, its maximum absorbance shifts to 595 nm c. the reducing effects of Coomassie Blue shifts the protein's maximum absorbance to 595 nm d. myoglobin is best observed at 595 nm
When we perform Bradford Assay to measure protein concentration, the spectrophotometer is set 595 nm because: a. the excitation peak of Coomassie Blue is best observed at 595 nm b. when Coomassie Blue binds to proteins, its maximum absorbance shifts to 595 nm c. the reducing effects of Coomassie Blue shifts the protein's maximum absorbance to 595 nm d. myoglobin is best observed at 595 nm
Organic Chemistry: A Guided Inquiry
2nd Edition
ISBN:9780618974122
Author:Andrei Straumanis
Publisher:Andrei Straumanis
ChapterL4: Proton (1h) Nmr Spectroscopy
Section: Chapter Questions
Problem 4CTQ
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- When we perform Bradford Assay to measure protein concentration, the spectrophotometer is set 595 nm because:
|
a. |
the excitation peak of Coomassie Blue is best observed at 595 nm |
|
b. |
when Coomassie Blue binds to proteins, its maximum absorbance shifts to 595 nm |
|
c. |
the reducing effects of Coomassie Blue shifts the protein's maximum absorbance to 595 nm |
|
d. |
myoglobin is best observed at 595 nm |
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