When you try separating an unknown mixture by normal-phase liquid chromatography with 50% hexane and 50% methyl t-butyl ether, the peaks are too close together. (not resolved) Should you use a higher or lower concentration of hexane in the next run?
When you try separating an unknown mixture by normal-phase liquid chromatography with 50% hexane and 50% methyl t-butyl ether, the peaks are too close together. (not resolved) Should you use a higher or lower concentration of hexane in the next run?
Chapter34: Miscellaneous Separation Methods
Section: Chapter Questions
Problem 34.1QAP
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1) When you try separating an unknown mixture by normal-phase liquid chromatography with 50% hexane and 50% methyl t-butyl ether, the peaks are too close together. (not resolved) Should you use a higher or lower concentration of hexane in the next run?
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