Suppose that you try separating an unknown mixture by normal-phase chromatography with the solvent mixture 50% hexane-50% methyl t-butyl ether (which is more polar than hexane). The peaks are too close together and are eluted rapidly. Should you use a higher or lower percentage of hexane in the next run?
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Suppose that you try separating an unknown mixture by normal-phase chromatography with the solvent mixture 50% hexane-50% methyl t-butyl ether (which is more polar than hexane). The peaks are too close together and are eluted rapidly. Should you use a higher or lower percentage of hexane in the next run?
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- Gas chromatography cannot analyze mixtures due to the sensitivity of the instrument. True False1a) Hexane, CH3(CH2)4CH3, is found to have a long retention time when separated from a mixture. A column with a C18 (18-carbon chain) stationary phase and a methanol-water modal phase is used for the separation. Explain why hexane has a long retention time. 1b)You wish to chromatographically seperate a mixtureof the follwing compunds. Which of the two compounds will be most difficult to seperatate from one another? Why?A scientist wishes to measure the concentration of methyl benzoate in a plant stream by gas chromatography. He prepares a sample of butyl benzoate to use as an internal standard. The results of a preliminary run, which used a solution known to contain 1.31 mg/mL of methyl benzoate (peak A) and 1.44 mg/mL of butyl benzoate (peak B), are shown. The area of peak A is determined to be 352 and the area of peak B is determined to be 395 measured in arbitrary units by the computer. To measure the sample, 1.00 mL of a standard sample of butyl benzoate containing 2.21 mg/ml is mixed with 1.00 mL of the plant stream material. Analysis of the mixture gave a peak area of 473 for peak A and 421 for peak B. What is the concentration of methyl benzoate in the plant stream? methyl benzoate concentration: 2.7 incorrect Detector Response 10 Time (min) 15 mg/mL
- A scientist wishes to measure the concentration of methyl benzoate in a plant stream by gas chromatography. He prepares a sample of butyl benzoate to use as an internal standard. The results of a preliminary run, which used a solution known to contain 1.37 mg/mL of methyl benzoate (peak A) and 1.51 mg/mL of butyl benzoate (peak B), are shown. The area of peak A is determined to be 3.40×102 and the area of peak B is determined to be 398 measured in arbitrary units by the computer. To measure the sample, 1.00 mL of a standard sample of butyl benzoate containing 2.49 mg/mL is mixed with 1.00 mL of the plant stream material. Analysis of the mixture gave a peak area of 437 for peak A and 415 for peak B. What is the concentration of methyl benzoate in the plant stream?A scientist wishes to measure the concentration of methyl benzoate in a plant stream by gas chromatography. He prepares a sample of butyl benzoate to use as an internal standard. The ic results of a preliminary run, which used a solution known to contain 1.37 mg/mL of methyl benzoate (peak A) and 1.51 mg/mL of butyl benzoate (peak B), are shown. The area of peak A is determined to be 301 and the area of peak B is determined to be 349 measured in arbitrary units by the computer. To measure the sample, 1.00 mL of a standard sample of butyl benzoate containing 2.41 mg/mL is mixed with 1.00 mL of the plant stream material. Analysis of the mixture gave a 10 Time (min) 15 peak area of 499 for peak A and 417 for peak B. What is the concentration of methyl benzoate in the plant stream? methyl benzoate concentration: mg/mL Detector ResponseIn chromatography, the compound that is least soluble in the mobile phase a) Travels the furthest away from the line of origin b) Stays closest to the line of origin
- A scientist wishes to measure the concentration of methyl benzoate in a plant stream by gas chromatography. He prepares a sample of butyl benzoate to use as an internal standard. The results of a preliminary run, which used a solution known to contain 1.35 mg/mL of methyl B benzoate (peak A) and 1.49 mg/mL of butyl benzoate (peak B), are shown. The area of peak A is determined to be 319 and the area of peak B is determined to be 381 measured in arbitrary units by the computer. To measure the sample, 1.00 mL of a standard sample of butyl benzoate containing 2.27 mg/mL is mixed with 1.00 mL of the plant stream material. Analysis of the mixture gave a peak area of 10 Time (min) 15 465 for peak A and 397 for pcak B. What is the concentration of methyl benzoate in the plant stream? methyl benzoate concentration: mg/mL Detector ResponseFrom a 10-mL sample, a 1-mL aliquot was taken and diluted to 100mL. From this, a 5-mL aliquot was taken and diluted to 20mL.The final 20mL was found to have a concentration of 0.004M analyte X.What is the concentration of analyte X in the 10-mL sample?6. A certain mixture contains the following compounds: propionic acid, 2-hexanol, isoamyl acetate, and 3,4-dimethylheptane. Rank the compounds in the order of decreasing rate of elution using normal phase liquid chromatography. Explain your answer.
- Chemistry Suppose you are trying to separate a mixture of compounds using reversed-phase liquid chromatography (RPLC). On the first run, using a mobile phase containing 40% tetrahydrofuran and 60% water, the peaks all elute between 0 and 3 min and are too close together to be quantitatively resolved. To improve the resolution of the peaks, should you increase or decrease the amount of tetrahydrofuran in the mobile phase for the next run? Incorrect. decrease increase The solvent tetrahydrofuran is less polar than water. In RPLC, a nonpolar stationary phase and a polar solvent is used for the separation. Eluent strength of the mobile phase increases as the polarity decreases. Decreasing the eluent strength of the mobile phase will result in an increase in the retention time and improved resolution of the compounds. not enough information In a separate experiment, you are trying to separate a mixture of compounds using normal-phase liquid chromatography (NPLC). On the first run, with a…A student developed a new dye called Dye C. It was analyzed against other dyes using paper chromatography. A solution of 1:1 ethyl acetate:hexane was used as the eluent. The resulting chromatogram is shown in the figure. Determine the retention factor (Rf) values of each spot on dye C. (use 2 decimal places) What other dye/s are present in dye C and what is the dye with least number of pigments?In all forms of chromatography, there is a mobile phase and a stationary phase. In thin layer chromatography what is the stationary phase?