Q: How do you make liquid bacteria culture?
A: It is important for growing more numbers of bacteria to isolate enough plasmid DNA for experimental…
Q: What is the major difference between animal cell culture and plant cell culture.
A: Cell culture methods used to cultivate cells in the laboratory include animal cell culture and plant…
Q: List the stages involved in preparing a specimen for observation under the light microscope.
A: All microscopes are designed to include a stage where the specimen usually mounted onto a glass…
Q: Enumerate the advantages derived from staining bacteria.
A: BASIC INFORMATION BACTERIA It is one of the type of biological cell. They belong to prokaryotic…
Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…
A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…
Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…
A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…
Q: What kind of medium might you make to selectively grow a bacterium that lives in your gut?
A: MacConkey agar is used as a selective and differential media for differentiating gut bacteria which…
Q: Find out what the various components of the medium used for propagation of an explant in vitro are?
A: Plant tissue culture is a technique through which plant cells, tissues or plant parts are grown on…
Q: How does colony morphology help you monitor the purity of your culture? What are disinfectants and…
A: Microbiology is the study of those living organisms which cannot be seen through naked eyes…
Q: How does the plant cell wall react when it is placed under hypotonic medium?
A: The plants are the multicellular eukaryotic organism of the kingdom Plantae. It is the autotrophic…
Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…
A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…
Q: What are the 4 types of culture?
A: Biological cultures include common laboratory means, which are involved in studying living organisms…
Q: What is secondary cell culture?
A: Secondary culture is a subculture of the primary culture. All the cells grown in the Petri dish…
Q: You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to plate out 100 cells…
A: We shall be performing a dilution calculation for this question. We know, C1V1 = C2V2 C1 is the…
Q: Which of the microscope objectives is most satisfactory for studying bacteria? Why?
A: For studying bacteria , under the microscope , 100X objective oil immersion lens is generally used…
Q: discuss the significance of the different aseptic techniques utilized in maintaining sterility of…
A: The laboratory that carries out growth of prokaryotic cells or eukaryotic cells in physiological…
Q: Why are bacterial cells generally stained for microscopic viewing?
A: Staining bacterial cells prior to microscopic viewing is a routine process in most of microbiology…
Q: Why must primary cell cultures be restarted every so often when preparing primary cell cultures to…
A: Primary cell cultures are derived from tissue and they are more similar to the in vivo state and…
Q: Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture…
A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…
Q: What are types of cell culture media?
A: A cell culture medium is solid or liquid in nature. It is a mixture of all necessary nutrients…
Q: Why is it important to perform a gram stain for any type of bacteria? After performing the gram…
A: The gram staining is a procedure that is useful in classifying the bacteria based on retention of…
Q: What external bacterial cell structures can be demonstrated by a negative stain?
A: The negative stain is useful in studying the morphology of bacterial cells. It also characterize…
Q: What type of agar would you use to grow gram positive bacteria
A: Bacteria are different from the plant as well as animals. They require specific substances in the…
Q: What is the purpose of using a biological stain when microscopically examining cellular components?
A: Staining is a technique that is adopted to enhance the visibility of the specimens under study that…
Q: What is the difference between conventional optical microscopes and inverted microscopes?
A: Introduction Conventional optical microscopes:- This type of microscope uses visible light (400-700…
Q: How is the correct way of incubating culture plate media? Explain why is that so.
A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…
Q: Why must materials and instruments used in tissue culture be kept sterile?
A: Sterilization of explants is a key step in plant tissue culture.
Q: What is the most important instrument in an animal cell culture laboratory?
A: The process by which animal, insect or human cells are isolated from a living tissue and grown in…
Q: Beginning with 10 grams of plant tissue, order the following procedures (as steps 1-4) that you will…
A: Cell organelles extraction The process to obtain cell organelles from the cell.
Q: Provide three applications of cell suspension culture
A: Suspension culture is a type of culture in which single cells or small aggregates of cells multiply…
Q: What are the advantages of solid media over liquid media?
A: Solid media-It is a growth medium which forms solid support for the growth of colonies of…
Q: Explain the relationship between bacterial cell wall with gram-positive and gram-negative in the…
A: Gram staining is the standard laboratory practice for distinguishing between two broad species of…
Q: Why must young cultures be used when doing a Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: laboratory? explain with diagram
A: The tissue culture is the general term for the removal of the cells, tissues or organs from an…
Q: What technology is available for faster bacterial culture?
A: BASIC INFORMATION BACTERIA It is one of the type of biological cell. They belong to prokaryotic…
Q: Which of the following methods used to enumerate cells often requires specialized staining to…
A: A growth medium is intended to promote the growth of a variety of microorganisms or cells by the…
Q: When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies…
A: Dilution is the process of decreasing the concentration of the solution. The cells count decreases…
Q: What are the advantages and disadvantages of using the Slide Culture technique?
A: Slide culture technique is mainly utilized for the preparation of fugal colonies. Fungi are…
Q: What is a step-by-step process of preparing and inspecting a microorganism under a microscope?…
A: In dark-field microscopy, microbes appear light in a dark background., viruses, macromolecules,…
Q: bacteria require staining before microscopic examination?
A: Bacteria are single-celled organisms with a small size. Bacteria can be found practically everywhere…
Q: what are the reagents for the four major steps of the gram stain procedure
A: Gram staining protocol is used to differentiate between Gram-positive and Gram-negative bacteria by…
Q: What are the advantanges and disadvantages of using stained preparations for observing microbial…
A: Characteristics of microbes are studied using a microscope by microbiologists. Some microbes like…
Q: What is the principle of Grams staining based on the cell wall composition of bacterial cell.
A: Bacteria are a kind of organic cell. They comprise an enormous area of prokaryotic microorganisms.…
Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?
A: Introduction We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…
Q: outline a protocol of cell culture techniques from call thawing to incubating.
A: Cryopreservation is a type of cell culture in which cells are freezer and stored. Cryopreservation…
Q: Define the meaning of Suspension cell culture
A: Cell culture is a media which is used for growth of the cells.
Q: How to prepare an agar plate for bacteria? How to include the antibiotics into agar plates? How long…
A:
Q: Elaborate on the advantages of examining colony in an agar plate.
A: An agar plate is a Petri dish which have a development medium set with agar, utilised to culture…
Q: What are the differences between “primary cell culture” and “continuous cell lines”?
A: Primary cell culture is the culture of cells directly isolated from parental tissue of interest.…
Q: What are the advantages and disadvantages of liquid media?
A: Microbiological culture is a method of multiplying microorganisms in lab conditions on growth media.
What should be considered when preparing a monolayer cell culture?
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- How to establish state for tissue culture in laboratory? explain with diagramExplain about different types of bioreactors and how they are used in cell suspension culture. What are the advantage and limitations of each bioreactors?What is the most important instrument in an animal cell culture laboratory?
- What are types of cell culture media?1)What is the natural color of cytoplasm? How is it related to our need to stain bacterial cells? 2) Which of the following is the main goal when staining a culture? A) improve contrast B) identify bacteria C) Visualize bacterial structuresWhat are the 4 types of culture?