Q: What is the difference between a mixed culture and a contaminated culture if they both contain more…
A: In microbiology , a bacterial culture is a growth of microorganisms which have been grown in a…
Q: Why don't you get isolated colonies from the pour plate if the broth culture was not diluted first?
A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…
Q: Can culture media be sterilized in the hot air sterilizer? Why or why not?
A: Hot air sterilization is a procedure in which the sterilization of the objects are done using hot…
Q: Why is it important to keep the culture at the correct incubation temperature when measuring the…
A: Generation time is the time which is taken by the bacteria to double in number during a specified…
Q: What are the disadvantages of doing a total microbial count by examining wet mounts under the…
A: Ans- Wet mounts have the following drawbacks: The benefit of being able to observe movement can also…
Q: Noah wanted to transfer Staphylococcus aureus from a broth to an agar plate. He picked up the broth…
A: The aseptic techniques are applied in the laboratory setup to reduce the risk of contamination. It…
Q: Indicate the correct way of inoculating the following media. Plate Media For sensitivity testing…
A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…
Q: What are pure cultures and why are they important? How are spread plates, streak plates, and pour…
A: In science and related fields, a culture plate is a low level lined lab holder for growing a layer…
Q: Why are semi-solid media such as TTC deeps suitable to test motility of bacteria?
A: There are several parameters which need to be studied in microbiology about different…
Q: why it is useful to plot the microbial growth from a growing microbial culture?
A: The term microbial growth refers to the growth of a population of microbe, it is an increase in the…
Q: Can you please discuss the principles of using Motility Test Medium for the detection of bacterial…
A: Bacterial motility is usually demonstrated in a semi-solid agar medium. Motile bacteria ‘swarm' in…
Q: Why after a certain period the growth rate of the culture decreases until growth stops?
A: Warmth, dampness, pH levels and oxygen levels are the four major physical and chemical components…
Q: What is the purpose of the quadrant streak plate method? - How could this method be used in a…
A: In quadrant streak plate method, first of all the plate on which bacteria has to be grown that is on…
Q: Why have several specialized media been developed? Give an example ofone such medium.
A: Culture media is of basic importance for many microbiological tests: to get pure cultures, to grow…
Q: The factors for the manipulation of culture medium?
A: Culture medium It is also called a growth medium. It is a solid, liquid, or semi-solid medium that…
Q: What is differential medium and differential media? Explain the difference ?
A: Difference between differential medumium - .- A differential medium support the expansion of any…
Q: What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of…
A: Microbiology is a branch of science which deals with the study of microscopic organisms such as…
Q: How is agar in the petri dish prepared to grow the bacterial specimen? explain in your own words.
A: Agar is a polymer made up of subunits of sugar galactose and is a component of cell walls of several…
Q: Why is mordant necessary for the staining of bacterial flagella? Answer in not more than 3…
A: In contrary to negative staining, which leaves the bacteria unstained against a dark backdrop,…
Q: Oven sterilization of aqueous culture medium. Effect: Rationale:
A: Oven sterilization utilizes dry heat of about 160 or 170 degrees celsius for one hour or 30 minutes,…
Q: You have a broth medium that you would like to turn into a solid medium in order to do streak…
A: The "nutrient broth" is essentially nutrient agar without the solidifying agent. They stay liquid at…
Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…
A: Preparation of culture media and inoculation are two of the very sensitive steps in any…
Q: What is a pure culture and how can one be obtained
A: Cells are the structural and functional units of life.
Q: During media preparation, you observed that the agar medium did not solidify after sterilization…
A: Nutrient Agar Medium is the artificial media which is used to grow microbes. It provides essential…
Q: What is differential medium
A: Differential medium or indicative media contain compounds which support the growth of microbes and…
Q: When labeling culture tubes or plates why do we use a pencil instead of a pen? Are there other…
A: Appropriately labeling specimens for research labs is of critical importance.
Q: How would you expect the staining properties of 24-hour culture of Bacillus subtilis or the other…
A: Bacillus subtilis is a very well bacteria that is widely used as a Gram-positive bacteria model…
Q: What are the advantages and disadvantages of using the Wet Mount technique?
A: As per bartleby guidelines, we are suppose to answer only one question kindly post other separately.…
Q: What is the name of this media? What type of media is it? What does the yellow color indicate? What…
A: Selective media generally selects for the growth of a desired organism, stopping the growth of or…
Q: You collected a sample from a patient with a suspected STI. After Gram staining, you observed this…
A: The staining of bacteria is an important method of classifying the bacteria There could be multiple…
Q: Are TSA, MRS and MOX differential media? Why or why not?
A: In microbial biology, differential media refers to any growth media that comprises of certain…
Q: In the preparation of a pure culture media, the petri plates are inverted when placed in an…
A: In microbiology, culture media is considered the growth medium that possesses the specific mixtures…
Q: Can culture media be sterilized in the hot air oven? why or why not?
A: Sterilization is the process of deactivating, killing, or removing any kind of microorganism (or…
Q: A novice microbiology student incorrectly explains that immersion oil increases the magnification of…
A: The function of immersion oil is to increase the resolving the power of the light microscope. The…
Q: Why does saliva not serve as a good microbialgrowth medium?
A: Saliva is the watery, frothy substance that is produced in the mouths of some animals, including…
Q: Distinguish batch culture and continuous culture
A: Fermentation is used to convert carbohydrates into alcohol or acids. This process occurs in some…
Q: With liquid media such as broth, is it possible to determine if the culture is pure using the naked…
A: A culture is a method by which microbes are allowed to reproduce and multiply in predetermined…
Q: What is the main difference between plates before and after centrifugation? What may be the purpose…
A: Answer: CENTRIFUGATION = It is the process involving mechanical force that is centrifugal force,…
Q: What is the nature of samples that should NOT be placed inside the drying-oven?
A: According to the question, we have to explain the nature of the samples that should not be placed…
Q: What is the major difference between an enrichment culture and a selective culture?
A: Culture media is used for cultivating microorganisms. Both selective media and enriched media are…
Q: What is the function of a mordant, and which reagent serves this purpose in the Gram stain…
A: An extensively used microbiological method of staining is called a Gram stain. It significantly…
Q: Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar…
A: # Here I am giving solution of first two questions . Please repost your third question and better to…
Q: What are the culture media that can be used for cultivating non-specific and specific…
A: A microbiological culture medium is a substance that promotes the growth, support, and survival of…
Q: What type of agar would you use to grow gram negative bacteria
A: Gram-negative bacteria are the bacteria that do not consist of the crystal violet stain and they are…
Q: Why does a streaking method used to inoculate plates result in isolated colonies? (Explain…
A:
Q: Which of the following types of media would be appropriate to use in order to culture a fastidious…
A: Answer:- From all the four above-mentioned medias yeast extract media and nutrient media are…
Q: Can pure culture be prepared directly from a mixed culture? Explain your answer.
A: A microbiological culture, also known as a microbial culture, is a way of multiplying microbial…
Q: Is the mannitol salt agar (MSA) a complex or defined medium? Explain based on Composition. What kind…
A: The selective ingredients of MSA the bile salts and the dye, crystal violet which inhibit the growth…
Q: If you were using the quadrant streak plate method to plate a very dilute broth culture (with many…
A: Streak plate technique is the technique or method that is widely used for the isolation of a pure…
Q: What are the primary uses of the following preparations of culture media? Agar regular slant Agar…
A: Hello. Since your question has multiple sub-parts, we will solve the first three sub-parts for you.…
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- What is the main difference between plates before and after centrifugation? What may be the purpose of concentrating the media and perform a second plating?Why should bacterial culture plates be incubated inverted (bottom up)?What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?
- What type of media are culture medium 1 and culture medium 2? And its difference to each other?Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms? Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?What is the difference between culture-based technique and culture-independent techniques based on the principle behind the method?
- What are the advantages and disadvantages of using the Wet Mount technique? What are the advantages and disadvantages of using the Hanging drop technique? What are the advantages and disadvantages of using the Slide Culture technique? pls elaborate each, thank youWhy should culture media after inoculation be incubated at an optimal temperature immediately?In the Harada-Mori culture technique, how are you going to dispose the culture tubes? Is it suitable to use refrigerated samples for this procedure Why or Why not?
- Why go to the trouble of creating a master plate (why not simply plate the initial culture on both nutrient agar and glucose-salts agar)?If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the pre-made plates by the time of the laboratory schedule?Why might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?