What phi and psi angles would you predict to be most prevalent for myoglobin and green fluorescent protein, in a Ramachandran plot
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- Download BLOSUM30 and BLOSUMB0 substitu- tion matrices and place them side by side on your computer screen. What are the differences between the two matrices? Why do you see these differences?In STR typing, the homozygous condition will create a higher peak than the heterozygous condition in terms of relative fluorescence. How do the capillary electrophoresis and the electropherogram correlate?why does a GC rich (70-80%) and GC poor (30-40%) give a low read coverage?
- In this problem, you will design a filter system for a flow cytometer. The four fluorescent dyes are FITC,PE, PE-TR,and PE-Cy5. Their emisison spectra are provided below. Detectors are arranged as shown in thefigure below. The FITC signal is detected from Filter 1, the PE signal from Filter 2, the PE-TR signal fromFilter 3 and the PE-Cy 5 signal from Filter 4. Part 4.1Provide a rationale for the order in which the filters are arranged with respect to the unfiltered signal. Part 4.2You are given 9 candidates from which you have to choose 4 for the filters in the diagram above. Choose4 and justify your selection.# Filter type WavelengthA Long pass 650 nmB Short pass 530 nmC Long pass 490 nmD Band pass 550-590 nmE Long pass 560 nmF Short pass 640 nmG Long pass 620 nmH Band pass 620-640 nmI Short pass 590 nmWhen comparing MS2 spectrums of various bacteria, what specifically is it that you are looking for? Why are the peaks different and what does that indicate between different strains of bacteria?Electrophoretic Analysis of DNA via Agarose gel Electrophoresis determine the voltage used (how to determine the voltage? Hint: check on volt/cm).
- Immunofluorescence and Fluorescent livecell imaging techniques can both be used to determine protein localisation.List the advantages and disadvantages of using immunofluorescence andfluorescent live cell imagingAlkaline phosphatase conjugated to the anti-DIG antibody recognizes the Dig-labeled probe. The enzymes cleaves the phosphate bond of the CDP-star substrate, resulting in the release of 4 photons captured on light-sensitive film. True or FalseConsider a batch culture of sphere-shaped bacteria in a growth medium or broth, in which the mean cell diameter of the bacteria is 2.0 μm (micron). Show all your calculations and assumptions in answering the questions below: What is the volume of one of these sphere-shaped cells, expressed in liters? If there are 3 x 10^12 cells in one liter of this culture broth, what percentage of the volume is occupied by bacteria and what percentage is occupied by the cell culture broth (water only)? If the cells contain 80% water, what is the dry cell concentration, expressed as “grams dry weight/Liter of broth”? You should assume that the density of the cells dry mass is approximately 1.0 gr/cm3
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