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What is the purpose of adding sodium bicarbonate to a culture medium?
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- What advantages do solid media offer for the isolation of microorganisms? How will you check the purity of an isolate?What is the importance of pH adjustment in culture media?What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?
- What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter culture media for a concentration of 6.5 x 106 cells/mL?What is diluent? Why is it useful for working with bacterial samples?How is the correct way of incubating culture plate media? Explain why is that so.