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Is it important to have fewer phages than bacterial host cells when doing a quantitative plaque assay? Explain.
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- What is the role of the following in the alkaline plasmid screen? G buffer (Cell Suspension Solution) Denaturing Solution (Cell Lysis Solution) Neutralization SolutionIn a mixed infection assay between h+r+ and hr phage, where h and r are two genes that govern plaque morphology, which of the following do you expect to observe? only h+r+ and hr only h+r and hr+ only h+r+ only hr All four types: h+r+, hr, h+r, hr+During the incubation step of the plaque assay, viral replication leads to host cell lysis. This allows the freshly-released virions to diffuse through the media and infect new hosts. The amount of agar added to the top agar is 7 g per liter rather than the 16 g of agar per liter for most solid media, including the bottom agar used in this assay. How would it change the plaque assay results if bottom agar was used in place of the top agar in this assay? a- The diameter of the plaques would be smaller. b- The diameter of the plaques would not change. c- The diameter of the plaques would be larger. (2) A researcher measured the viral titer of PhiX174 in fish tank water, untreated sewage water, and unpasteurized apple juice. Which sample do you predict will have the highest titer on this page? a- Fish tank water b- Unpasteurized apple juice c- Untreated sewage water
- What is the purpose of including a tube in the phage assay in which only the E. coli culture is inoculated? Explain.Which of the following is TRUE when one assay bacteriophage titers? You should: a) first mix the phages with a live bacterial culture and then pour the mixture on the agar plate b) directly add the phage dilution onto the surface of an agar plate c) add tryptic soy broth to the phage dilution and incubate overnight d) incubate a phage solution with live bacterial cells for several minutes. You must add soft agar to the mixture before pouring the content on the agar plateWhat is the purpose of using Triton X in hemolytic assay.
- The strain of λ phage t is cI857. That tells you that the cI DNA segment is disabled by a specific mutation. What is the exact genetic change in cI857? What specific property of the cI gene product does this mutation change, and how does this help titering for a plaque assay?what are some advantages to using latex particles instead of blood cells for IM assay?What is an advantage of using an ELISA instead of a protein microarray to study a proteome? What is a disadvantage?
- Based on the image below of a Kirby-Bauer assay, this bacteria is most susceptible to which antibiotic? Write the letter of the antibiotic below. G. B. E.Explain: Describe an electrochemical sensor assay method for rapid bacterial detection and identification. What are the principles and mechanisms involved? functionalization of a sensor array with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase linked DNA oligonucleotide detector probe. Explain how bacteria are being detected using an electrochemical sensorExplain why gene mapping is important and how this technique can be used in diseaseinvestigation? (Answer within 250-500 words only)