v. (mg min (with threo-sphingosine) Sphingosine (иМ) v. (mg - min) (no inhibitor) 2.5 32.3 8.5 3.5 40 11.5 5 50.8 14.6 72 10 25.4 43.9 20 87.7 115.4 50 70.8 Construct a Lineweaver-Burk plot to answer the following questions: (a) What are the apparent KM and Vmax Values in the presence and absence of the inhibitor? (b) What kind of an inhibitor is threo-sphingosine? Explain

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter9: Parenteral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 6.4P
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Sphingosine-1-phosphate (S1P) is important for cell survival. The synthesis of S1P from sphingosine and ATP is catalyzed by the enzyme sphingosine kinase. An understanding of the kinetics of the sphingosine kinase reaction may be important in the development of drugs to treat cancer. The velocity of the sphingosine kinase reaction was measured in the presence and absence of threo-sphingosine, a stereoisomer of sphingosine that inhibits the enzyme. The results are shown below.

Construct a Lineweaver-Burk plot to answer the following questions: (a) What are the apparent KM and Vmax values in the presence and absence of the inhibitor? (b) What kind of an inhibitor is threo-sphingosine? Explain.

v. (mg min
(with threo-sphingosine)
Sphingosine
(иМ)
v. (mg - min)
(no inhibitor)
2.5
32.3
8.5
3.5
40
11.5
5
50.8
14.6
72
10
25.4
43.9
20
87.7
115.4
50
70.8
Construct a Lineweaver-Burk plot to answer the following questions:
(a) What are the apparent KM and Vmax Values in the presence and absence of the inhibitor?
(b) What kind of an inhibitor is threo-sphingosine? Explain
Transcribed Image Text:v. (mg min (with threo-sphingosine) Sphingosine (иМ) v. (mg - min) (no inhibitor) 2.5 32.3 8.5 3.5 40 11.5 5 50.8 14.6 72 10 25.4 43.9 20 87.7 115.4 50 70.8 Construct a Lineweaver-Burk plot to answer the following questions: (a) What are the apparent KM and Vmax Values in the presence and absence of the inhibitor? (b) What kind of an inhibitor is threo-sphingosine? Explain
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