In a normal-phase liquid chromatographic column, a solute was found to have a retention time of 26.7 minutes, and an unretained component required 0.55 minutes for elution when the mobile phase was 40% (by volume) THF and 60 % acetonitrile. Calculate: a) Retention factor (k) b) the THF-acetonitrile composition that should bring k to a value of 4.
In a normal-phase liquid chromatographic column, a solute was found to have a retention time of 26.7 minutes, and an unretained component required 0.55 minutes for elution when the mobile phase was 40% (by volume) THF and 60 % acetonitrile. Calculate: a) Retention factor (k) b) the THF-acetonitrile composition that should bring k to a value of 4.
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter26: An Introduction To Chromatographic Separations
Section: Chapter Questions
Problem 26.14QAP
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In a normal-phase liquid chromatographic column, a solute was found to have a retention time of 26.7 minutes, and an unretained component required 0.55 minutes for elution when the mobile phase was 40% (by volume) THF and 60 % acetonitrile.
Calculate: a) Retention factor (k)
b) the THF-acetonitrile composition that should bring k to a value of 4.
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