Dr. Wakefield would like to isolate recombinant plasmids from her bacterial culture using thealkaline lysis method. She is planning to use the chemicals as listed below:Solution I: 50 mM glucose, 25 mM Tris-Cl (pH 8.0), 10 mM EDTA (pH 8.0)Solution II: ???Solution III: 5 M potassium acetate, glacial acetic acid, de-ion waterEthanol 70% (v/v)IsopropanolTE-RNAase pH 8.0(i)(ii)(iii)Based on the chemical list above, state the content(s) of Solution II.Explain the functions of Solution II described in Q3 a) (i) in plasmid isolation.What is the role of alcohol precipitation conducted after the plasmids are obtained atthe end of the procedure? Discuss the roles of ethanol and salt in alcohol precipitationstep.

Judging by the Chemical reagents used in the protocol ,it is alkaline lysis method of Plasmid…

Solution I: 50 mM glucose, 25 mM Tris-Cl (pH 8.0), 10 mM EDTA (pH 8.0) Solution II: ??? Solution III: 5 M potassium acetate, glacial acetic acid, de-ion water Ethanol 70% (v/v) Isopropanol TE-RNAase pH 8.0 (i) (ii) Based on the chemical list above, state the content(s) of Solution II. Explain the functions of Solution II described in Q3 a) (i) in plasmid isolation.

Solution I: 50 mM glucose, 25 mM Tris-Cl (pH 8.0), 10 mM EDTA (pH 8.0) Solution II: ??? Solution III: 5 M potassium acetate, glacial acetic acid, de-ion water Ethanol 70% (v/v) Isopropanol TE-RNAase pH 8.0 (i) (ii) Based on the chemical list above, state the content(s) of Solution II. Explain the functions of Solution II described in Q3 a) (i) in plasmid isolation.

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter3: Basic Hemostasis

Section3.6: D-dimers

Problem 4RQ

See similar textbooks

Similar questions

Why is polyacrylamide gel is used in electrophoresis?
Centrifugation is the first step in most fractionations, but it separates only components that differ greatly in size. Compare and contrast very high, high, medium, and low-speed centrifugations of supernatant mixtures. Define the following: SDS Polyacrylamide-Gel Electrophoresis and Immunoprecipitation
The images attached are the photos of bacteria in yoghurt under a misroscope. According to these images and your own knowledge, can you make a biological drawing of bacteria in yoghurt ( in a circle) and identify the types of bacteria accurately?
Gel used in electrophoresis is/are: a. Agarose gel b. Polyacrylamide plain gel c. Polyacrylamide SDS impregnated Polyacrylamide gel (Sodium dodecyl sulphate)
Which reagent removes contaminant proteins from DNA solution? Sodium dodecyl sulfate (SDS) Sodium citrate saline buffer Cold absolute ethanol O HCI
Since higher concentration colcemid will result in shorter chromosome, you want to change your protocol and reduce final concentration of colcemid in your 10 ml blood culture from 0.1ug/ml to 0.05ug/ml. how many ul colcemid stock solution with concentration10ug/ml needed to be added in 10ml blood culture?
A bacteriophage stock with a concentration of 1.45 x 1014 PFU/ml is prepared for an experiment. After dilution, 0.5 ml of the stock is plated and 100 plaques are found. What is the dilution factor used in this process? Answer in scientific notion with 3 significant digits.
A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This solution is serially diluted three times by combining 0.1 mL of each sequential dilution with 9.9 mL of liquid medium. Then 0.1 mL of the final dilution is plated in the plaque assay and yields 17 plaques. What is the initial density of bacteriophages in the original 1 mL?
A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This solution is serially diluted 3 times by combining 0.1 mL of each sequential dilution with 9.9 mL of liquid medium. Then 0.1 mL of the final dilution is plated in the plaque assay and yields 12 plaques.What is the initial density of bacteriophages in the original 1 mL? Enter your answer to two significant figures ( for example: 1.1 * 10^2)
The following pictures show the results of a Disk Diffusion Assay for different types of bacteria. For each bacteria, what antibiotic would you recommend be used on the patient? Explain your choice.
What are the importance of accurate wavelength measurement in purified DNA sample? Explain why contaminants must be avoided in DNA samples.
Identify the following on the gel electrophoresis 2-leg 2-log ladder 3 Kb 1022 bp 1022 bp 765 bp 880 bp 765 bp 1 Kb S00 hp 100 bp sample given