Blood agar is often used to observe changes in the appear-ance of the agar around the colonies growing on this medium.This medium could then be called:(a) Selective(b) Designated(c) Differential(d) Defined(e) Exact
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Blood agar is often used to observe changes in the appear-
ance of the agar around the colonies growing on this medium.
This medium could then be called:
(a) Selective
(b) Designated
(c) Differential
(d) Defined
(e) Exact
Step by step
Solved in 2 steps
- (1) why can't we say "sterile" technique (2) how are aseptic technique similar and different in the lab and Healthcare field?Be specific and explain at least 2 differences and two similarities. (3) You are asked to develop a method of transfer an unknown organism from a liquid broth to a solid petri dish.list each step that you would have to take .be specificA culture of Staphylococcus is diluted as follows:(1) 20mL are added to 80mL of water.(2) 10uL from (1) are then added to 9.99mL of water.(3) A 10-2 dilution is made from tube # (2).(4) 100uL from (3) are plated for a pour plate and incubated. There were 34 colonies counted on one quarter of the plate following incubation. a) What was the overall dilution?b) How many cfu/mL were present in the original culture?Two dyes wereplaced in an agar gel. Which dye migrated through thegel faster? Why? Explain in brief simply terms
- In preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?Using your fingers, you are asked to aseptically touch the surface of a sterile agar plate. Illustrate the possible result from this step if your fingers are (a) unwashed – touched various things prior to placing on agar surface, and (b) washed with soap or disinfected with 70% alcohol. Describe the relative abundance of microbial growth observed on the plates. List and draw the possible characteristics of an isolated bacterial colony that can be observed based on type of (a) margin, (b) elevation, (c) texture, and (d) optical property.Kligler’s iron agar and SIM are multiple test media.a. What physiological characteristic is detected by both of these media? b. What component of both media allows you to detect the preceding characteristic? c. Both media are stabbed but for different reasons. Explain.
- You just finished plating your electroporatio products on your YPD-Zeocin plate and you think you did everything perfectly but you come back the folloeing week and have zero colonies. Which of the following could be the reason for the absence of colonies? A) You centrifuged your electroporated cells for 30 sec at 16,000 rpm instead of 4,000 rpm before plating them B) You added sorbitol+YPD to your cells thirty minutes after pulsing your cells C) The Zeocin had not been added to the plates when they were made. D) All of the above E) Both a and b onlyduring inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.Gram straining procedure. Indicate the following information A. Shape........ B. Stained color under microscope........ C. PG Wall thick, thin or both.....
- Why is it important to always flame the mouth of a test tube or a petri plate in performing aseptic transfer techniques. In flaming a wire loop, why does it need to be red hot?There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one has been incubated for 24 hours. After a 10x dilution by taking 100µl of each culture and adding it to 900 µl water in a microcentrifuge tube and 100µl sample from the tube was taken to view in the counting chamber. a) Count the total number of yeast cells for each culture respectively b) Calculate the concentration and density of yeast cells for each culture respectivelyIn this experiment, a culture was serially diluted to the concentrations below. Each plate was plated with 0.25mL of the dilution. Using the most diluted plate, what is the correct concentration of the original culture? A) 2.0 X 10^-3 cells/mL B) 2.0 X 10^5 cells/mL C) 4.0 X 10^5 cells/mL D) 5.0 X 10^4 cells/mL