indicators, key used for/ determines reagents, or key positive result ingredients |1. crystal violet Comments/ Medium/test negative result additional info decolorization is key. Must include +/- controls on gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink 3. gram's declorizer 4. safranin shape every slide to verify result check this for any quadrant streak- isolation of pure culture of bacteria TSA pigmentation of colonies Eosin methylene blue agar (EMB) 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. blackening of medium 2. red color in added reagent 3. fuzzy appearance of medium/stab not visible 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. ferrous salts SIM 2. Kovac's in one medium) 3. low % agarose motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red 1. fermentation broth test media (list the specific carbs tested) phenylalanine agar urea agar or broth Methyl red test Vogues Proskauer test nitrate broth oxidase test casein agar gelatin deep lysine iron: lysine deaminase lysine decarboxylasse Pseudo. P agar Pseudo. F agar catalase test Mannitol Salt Agar Blood Agar with CAMP reaction Novobiocin sensitivity Bacitracin sensitivity bile esculin coagulase Lancefeild A Immunochromatograp hy test add any other additional tests here
indicators, key used for/ determines reagents, or key positive result ingredients |1. crystal violet Comments/ Medium/test negative result additional info decolorization is key. Must include +/- controls on gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink 3. gram's declorizer 4. safranin shape every slide to verify result check this for any quadrant streak- isolation of pure culture of bacteria TSA pigmentation of colonies Eosin methylene blue agar (EMB) 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. blackening of medium 2. red color in added reagent 3. fuzzy appearance of medium/stab not visible 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. ferrous salts SIM 2. Kovac's in one medium) 3. low % agarose motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red 1. fermentation broth test media (list the specific carbs tested) phenylalanine agar urea agar or broth Methyl red test Vogues Proskauer test nitrate broth oxidase test casein agar gelatin deep lysine iron: lysine deaminase lysine decarboxylasse Pseudo. P agar Pseudo. F agar catalase test Mannitol Salt Agar Blood Agar with CAMP reaction Novobiocin sensitivity Bacitracin sensitivity bile esculin coagulase Lancefeild A Immunochromatograp hy test add any other additional tests here
Chapter15: Antibiotic Agents
Section: Chapter Questions
Problem 20RQ
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