I have a 25x stock solution of buffer. How many ml do I add to water to make 50ml of 1x buffer O A. 1 ml 25x stock, 49 ml water B. 10 ml 25x stock, 40 ml water C. 2 ml 25x stock, 48 ml water D. 25 ml 25x stock, 25 ml water O E. 5 ml 25x stock, 45 ml water
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- Calculate the amount of BSA you will add to the tube to get the concentrations listed on the chart. Your stock tube is 2mg/mL. The amount of water will be the amount to bring your total volume up to 100uL. Standard Concentration BSA H2O Total volume A 200 ug/mL 100uL B 150 ug/mL 100uL C 100 ug/mL 100uL D 50 ug/mL 100uL E 20 ug/mL 100uL F 10 ug/mL 100uL G 5 ug/mL 100uL H 0 ug/mL 0 uL 100uL 100uLJoshua needs 150mL of 1X TBE buffer. The stock of TBE Buffer is 15X. How much 15X TBE buffer should he use to make 150mL 1X TBE buffer? O 5mL 25mL O 10mL O 15mL O 20mL O o o oa. calculate the volume of 0.1 M H2PO4– required to prepare 100 mL of your assigned buffer. b. calculate the volume of 0.1 M HPO42– required to prepare 100 mL of your assigned buffer. 1. Assigned pH Value ___6.90_________ 2. pKa Value of Assigned Buffer ___7.20_________ 3. Ratio 0.5011
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