Consider the TLC plate pictured below. What is the Rf of the compound if the compound distance is 2.77 cm and the solvent front distance is 5.85 cm? Please report your answer to 2 decimal places only!
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- If the TLC plates had been developed in a more polar solvent, would retention factors on the whole be larger, smaller, or unchanged? Briefly (1 - 2 sentences) explain your reasoningzero absorbance or 100 %T. If not, adjust the spectrophotometer accordingly. (2) Set the wavelength of the spectrophotometer to 400 nm. . Place the stock solution (Solution 5) in a cuvet following the "blank solution procedure" as above. Measure and record the wavelength and absorbance. Scan the wavelength range of the spectrophotometer while recording absorbance values. You will need to scan manually, setting the absorbance at zero (100% T) with the blank solution (0.1 M HNO3) at EACH chosen wavelength before recording the absorbance of the stock solution. Plot the data, absorbance, A (y-axis) versus the wavelength, λ (x-axis), to determine the λmax, the wavelength where maximum absorbance occurs for the cobalt ion. Absorbance 0.6 0.5 0.4 0.3 0.2 0.1 0.0 300 400 500. 600 Wavelength (nm) A representative absorption spectrum of a laboratory solution. 148 Page .700 (1) Absorbance of the Standard Solutions. 800 4 of 9 O - ZOOM +If the selectivity factor is equal to 1, the peaks are separated. True or False?
- As solvent polarity increases, the Retention Factor (Rf) of compounds on the TLC plate increases. Group of answer choices True FalsePlant pigments in spinach were separated with TLC with cellulose as thestationary phase and 80/20 diethyl ether/acetone mixture as themobile phase. If needed, the solvent structures in the lab Appendix.1. Determine the Rf values of each plant component on this TLC plate.2. Arrange the plant components from most polar to most nonpolar. Please answer both 1 and 2 (picture below is the same question attached )Calculate the Rr value for a spot in a TLC experiment if the solvent moved 14.5 cm and the spot moved 6.1 cm from the origin. Type answer:
- Record your absorbance data for the YELLOW and RED dyes below. Calculate the dye concentration in mM for each cuvette using M1V2 = M2v2 . Remember the concentration of stock solutions (100^ % dye) is 0.020 mM(mM= millimolar =mm /L). Data for YELLOW: peak absorbance used on spectrophotometer =420nm Cuvette 1 - 100% dye = 0.680 absorption Cuvette 2 - 75% dye = 0.510 absorption Cuvette 3 - 50% dye = 0.340 absorption Cuvette 4 - 25% dye = 0.170 absorption Data for RED - peak absorption used on spectrophotometer = 500nm Cuvette 1 - 100% dye: 0.621 absorption Cuvette 2 - 75%: 0.466 absorption Cuvette 3 - 50% dye: 0.311 absorption Cuvette 4 - 25% dye: 0.1555 absorptionWhat is the Rf value given the following information: Height of Chromatography Paper: 5.0 cm Distance from Bottom of Paper to Origin: 1.5 cm Distance from Origin to Spot: 1.6 cm Distance from Origin to Solvent Front: 2.9 cm Your Answer: AnswerIf the peak width w of both Peak 1 and Peak 2 fro eon 1g is 0.5 minutes, what is the resolution factor between the two peaks? 1.13 Selectivity is Peak 1 (6.0 min, Peak 2 w=0.5 (6.8 min, w=0.5 25 min) min) 20 15 Hold up peak (1.8 min) 10 0. 0. 4. 8. time (minutes) 2. 5. Signal