(2) Set the wavelength of the spectrophotometer to 400 nm. . Place the stock solution (Solution 5) in a cuvet following the "blank solution procedure" as above. Measure and record the wavelength and absorbance. Scan the wavelength range of the spectrophotometer while recording absorbance values. You will need to scan manually, setting the absorbance at zero (100% T) with the blank solution (0.1 M HNO3) at EACH chosen wavelength before recording the absorbance of the stock solution. Plot the data, absorbance, A (y-axis) versus the wavelength, à (x-axis), to determine the Amax, the wavelength where maximum absorbance occurs for the cobalt ion. Absorbance 0.6 0.5 0.4 0.3 0.2 0.1 0.0 500% 600 Wavelength (nm) A representative absorption spectrum of a laboratory solution. 148 300 400 700 800
(2) Set the wavelength of the spectrophotometer to 400 nm. . Place the stock solution (Solution 5) in a cuvet following the "blank solution procedure" as above. Measure and record the wavelength and absorbance. Scan the wavelength range of the spectrophotometer while recording absorbance values. You will need to scan manually, setting the absorbance at zero (100% T) with the blank solution (0.1 M HNO3) at EACH chosen wavelength before recording the absorbance of the stock solution. Plot the data, absorbance, A (y-axis) versus the wavelength, à (x-axis), to determine the Amax, the wavelength where maximum absorbance occurs for the cobalt ion. Absorbance 0.6 0.5 0.4 0.3 0.2 0.1 0.0 500% 600 Wavelength (nm) A representative absorption spectrum of a laboratory solution. 148 300 400 700 800
Chapter4: Least-squares And Calibration Methods
Section: Chapter Questions
Problem 9P
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