Complete culture medium is made of Select one: a. DMEM, 10% fetal bovine serum and 1% antibiotics/antifungal reagent b. DMEM and 0.25% trypsin c. 10% fetal bovine serum Od. DMEM is used to deactivate trypsin after it detaches cells. Select one: O a. warming up at 37 degree Celsius O b. 1x PBS O c. complete culture medium Od. DMEM The optimal temperature and pH to grow most mammalian cells are: Select one: O a. 37 degree C, 7.4 O b. 37 degree c, 6.8 O c. 32 degree C, 7.4 d. 98.6 degree C, 7.8

Complete culture medium is made of Select one: a. DMEM, 10% fetal bovine serum and 1% antibiotics/antifungal reagent b. DMEM and 0.25% trypsin c. 10% fetal bovine serum Od. DMEM is used to deactivate trypsin after it detaches cells. Select one: O a. warming up at 37 degree Celsius O b. 1x PBS O c. complete culture medium Od. DMEM The optimal temperature and pH to grow most mammalian cells are: Select one: O a. 37 degree C, 7.4 O b. 37 degree c, 6.8 O c. 32 degree C, 7.4 d. 98.6 degree C, 7.8

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter30: Assisting With Minor Surgery

Section: Chapter Questions

Problem 2CR

See similar textbooks

Similar questions

A phagehunter performs a full plate titer using standard techniques (100 ul of each dilution added to 250 ul of Gordonia terrae cells) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 72 plaques on the 10-7 dilution plate. Which of the following scenarios is the best choice for the phagehunter to do next? a.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation. b.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment. c.) The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments. d.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates.
Draw these steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 ml of 1X diluent or saline in a test tube. Pipet 50 µul of the diluted sample onto one test circle on the slide. 3. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.
Draw the steps of Qualitative Slide Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR: SCREEN AND TITER 1. Bring all reagents and test samples to room temperature before use. 2. Make a 1:20 dilution of the patient serum by pipetting 0.1 ml of patient serum into 1.9 mL of 1X diluent or saline in a test tube. Pipet 50 µl of the diluted sample onto one test circle on the slide. 3. Place one drop (about 50 mL) of positive control onto a separate test circle and one drop of negative control onto another test circle. 4. Invert the latex reagent bottle several times to mix the beads into the suspension. Add one drop of well-shaken reagent to each test area of the slide. 5. Mix each test area with a disposable stirrer, spreading each mixture over the entire section. Use a clean disposable stirrer for each test area. 6. Rock the slide gently with a rotary motion for 2 minutes and observe immediately for agglutination.
You need to prepare medium for your culture cells. Your salt solution is supplied at a 10X concentration but needs to be 2X for use. You also need to add fetal bovine serum for a final concentration of 8%. What would you add of each for the correct final concentrations in 5 L of media?
Discuss and explain the results of this graph. Graph shows results of WST-1 assay. T0 ( time zero) shows assay performed in order to obtain an absorbance at the time of test agent added in order to determine 1. how much cells have grown over the incubation period 2. Growth inhibition by test agent 3. If test agent caused cytotoxicity
Cells were incubated with chloroquine for 2 hours before stimulation with lipopolysaccharide (LPS, lg/ml). Cell viability was measured by staining cells with 3-(4,5-dimethylthiazol-2- yl)-2,5- diphenyltetrazolium bromide. TNF in the culture supernatant and the cell lysate were measured by sandwich ELISA, using specific monoclonal antibodies according to the manufacturer's instructions. To determine the level of cell-associated cytokines, cells were washed with phosphate-buffered saline (PBS) and lysed in a buffer containing 0.5% NP-40. Standard recombinant proteins were also diluted in PBS containing the same concentrations of NP-40 as the cell lysate. Cytokine synthesized were proteolytically cleaved and released into medium. The amount of cytokines in the culture supernatant and the cell lysate were presented. Results for TNF production are shown in Figure 1 (Jang et al, 2006). (Cells : PBMC: Human peripheral blood mononuclear cells, monocytes, monocytic lymphoma U-937 and leukemia…
At the station there is a 2 mL microcentrifuge tube containing sheep blood and 3 tubes containing the following solutions: 0 mM, 300 mM, 600 mM of sucrose. The tubes are randomly labelled A, B, C. 2 mL of each solution were transferred to a different microcentrifuge tube, 20 ul of blood were then added to each tube, and they were mixed well by inverting the tube for multiple times. They were observed and it was determined whether the solution in each tube was hypotonic (Yes or No) relative to the blood cells. In addition, the solutions were diluted with water to help you determine which solution is which sucrose concentration. What sucrose concentration corresponds with each tube? Tube Hypotonic (Yes/No) Hypotonic after 1:1 dilution with water (Yes/No) Most likely Sucrose concentration (mM) A No Yes B Yes Yes C No No
Which one of the following methods of antimicrobial susceptibility testing requires a dilution series of the antibiotic and can provide a minimum inhibitory concentration? Select one: a. Disk diffusion method b. Broth microdilution method c. Agar macrodilution method d. Gradient diffusion method
No zone of inhibition was seen on the Blood Agar Plate with the Optochin Test. How should the microorganism be characterized with Optochin? O This is nothing significant. The microorganism is resistant to Optochin. O The microorganism is sensitive to Optochin. O There is no change.
An inoculating loop or needle is sterilized using heata. by one brief passage.b. for exactly 5 minutes.c. until the entire wire is bright red.d. until the handle is bright red.e. until the tip is bright red.
Cells were incubated with chloroquine for 2 hours before stimulation with lipopolysaccharide (LPS, 1g/ml). Cell viability was measured by staining cells with 3-(4,5-dimethylthiazol-2- yl)-2,5- diphenyltetrazolium bromide. TNF in the culture supernatant and the cell lysate were measured by sandwich ELISA, using specific monoclonal antibodies according to the manufacturer's instructions. To determine the level of cell-associated cytokines, cells were washed with phosphate-buffered saline (PBS) and lysed in a buffer containing 0.5% NP-40. Standard recombinant proteins were also diluted in PBS containing the same concentrations of NP-40 as the cell lysate. Cytokine synthesized were proteolytically cleaved and released into medium. The amount of cytokines in the culture supernatant and the cell lysate were presented. Results for TNF production are shown in Figure1 (Jang et al, 2006). (Cells : PBMC: Human peripheral blood mononuclear cells, monocytes, monocytic lymphoma U-937 and leukemia…
The organism tested on Blood Agar (plate shown furthest to the left) is: a. Micrococccus luteus b. Staphylococcus aureus. c. Staphylococcus epidermidis You know because of which specific features of the hemolytic reaction