An enzymatic reaction was carried out in a batch-operated reactor until exhaustion of the substrate. The formation of the product was monitored over time, obtaining the results shown in Table 1. Answer: a) Knowing that the initial substrate concentration was 0.2 M and that the stoichiometry reaction is: 1S –> 2P, estimate the substrate concentration over time from the data from [P] data in Table 1; b) Determine the parameters of the Michaelis-Menten equation, Vmax and Km, by fitting direct from the hyperbola function; c) Determine the parameters Vmáx and Km, by the linearized equation;

Principles Of Radiographic Imaging: An Art And A Science
6th Edition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Chapter1: Basic Mathematics
Section: Chapter Questions
Problem 8RQ
icon
Related questions
Question

An enzymatic reaction was carried out in a batch-operated reactor until exhaustion of the substrate. The formation of the product was monitored over time, obtaining the results shown in Table 1. Answer:


a) Knowing that the initial substrate concentration was 0.2 M and that the stoichiometry reaction is: 1S –> 2P, estimate the substrate concentration over time from the data from [P] data in Table 1;


b) Determine the parameters of the Michaelis-Menten equation, Vmax and Km, by fitting direct from the hyperbola function;


c) Determine the parameters Vmáx and Km, by the linearized equation;

t (h)
[P] (M)
0,0
0,8
1,7
2,8
3,7
4,5
5,0
0,39
5,6
0,00
0,08
0,15
0,25
0,30
0,36
0,40
Transcribed Image Text:t (h) [P] (M) 0,0 0,8 1,7 2,8 3,7 4,5 5,0 0,39 5,6 0,00 0,08 0,15 0,25 0,30 0,36 0,40
Expert Solution
steps

Step by step

Solved in 5 steps with 3 images

Blurred answer
Recommended textbooks for you
Principles Of Radiographic Imaging: An Art And A …
Principles Of Radiographic Imaging: An Art And A …
Health & Nutrition
ISBN:
9781337711067
Author:
Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:
Cengage Learning
Principles Of Pharmacology Med Assist
Principles Of Pharmacology Med Assist
Biology
ISBN:
9781337512442
Author:
RICE
Publisher:
Cengage
An Illustrated Guide To Vet Med Term
An Illustrated Guide To Vet Med Term
Biology
ISBN:
9781305465763
Author:
ROMICH
Publisher:
Cengage