A mixture containing glutamic acid, arginine, phenylalanine and valine was subjected to anion exchange chromatography at pH 7.0. Which of the amino acids interacted with the resin (anion exchanger) and last to be eluted with a buffer of decreasing pH? A. Glu B. Val C. Phe D. Arg
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- Consider a buffer solution of acetate. The volume is 500 ml, the concentration is 200 mM, and the pH is 5.0. a. How many total moles of acetate plus acetic acid are present in the solution? Express answer as x.y with one digit before and one after the decimal place. b. What is the ratio of acetate ions (Ac-) to acetic acid ions (HAc) in the buffer solution (pH 5.0) if the pka is 4.76? Express the answer as x.yz with one digit before and two after the decimal place. c. How many moles of acetate are present in the solution? Express your answer to one decimal place. d. How many moles of acetic acid are present in the solution? Express your answer to one decimal place.An amino acid mixture consisting of lysine, leucine, and glutamic acid is to be separated by ion-exchange chromatography, using a cationexchange resin at pH 3.5, with the eluting buffer at the same pH. Which of these amino acids will be eluted from the column first? Will any other treatment be needed to elute one of these amino acids from the column?A dialysis bag containing a 3 M solution of the sugarfructose is placed in the following solutions. In each case,give the direction in which water flows.a. 1 M sodium lactateb. 3 M sodium lactatec. 4.5 M sodium lactate
- The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.If you have 50 ml of pure stock formalin solution how many ml of diluent will you need? What is the total volume of the reagent used you have prepared? Write down your answers with complete solutions and correct labels.The glucose content of samples can be determined using Nelson't test. The equation of the line for the standard curve preapred for varying concentrations of glucose is y = 3.0x + 0.2. Based from this information, determine the glucose concentration (in M) of a strawberry preserve having an absorbance reading of 1.35.
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